ZoBio B.V., BioPartner 2 Building, J.H. Oortweg 19, 2333 Leiden, the Netherlands.
Department of Biological Regulation, Weizmann Institute of Science, 234 Herzl Street, 761000 Rehovot, Israel.
J Magn Reson. 2019 Sep;306:202-212. doi: 10.1016/j.jmr.2019.07.018. Epub 2019 Jul 13.
Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for investigating cellular structures and their compositions. While in vivo and whole-cell NMR have a long tradition in cell-based approaches, high-resolution in-cell NMR spectroscopy is a new addition to these methods. In recent years, technological advancements in multiple areas provided converging benefits for cellular MR applications, especially in terms of robustness, reproducibility and physiological relevance. Here, we review the use of cellular NMR methods for drug discovery purposes in academia and industry. Specifically, we discuss how developments in NMR technologies such as miniaturized bioreactors and flow-probe perfusion systems have helped to consolidate NMR's role in cell-based drug discovery efforts.
核磁共振（NMR）光谱学是研究细胞结构及其成分的多功能工具。虽然基于细胞的方法在体内和全细胞 NMR 方面有着悠久的传统，但高分辨率的细胞内 NMR 光谱学是这些方法的新补充。近年来，多个领域的技术进步为细胞磁共振应用提供了汇聚效益，特别是在稳健性、可重复性和生理相关性方面。在这里，我们回顾了学术界和工业界中基于细胞的 NMR 方法在药物发现方面的应用。具体来说，我们讨论了 NMR 技术的发展，如微型生物反应器和流动探头灌注系统，如何帮助巩固 NMR 在基于细胞的药物发现工作中的作用。
