State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guizhou, People's Republic of China.
Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Guizhou Medical University, Guizhou, People's Republic of China.
Nat Prod Res. 2021 Apr;35(8):1307-1312. doi: 10.1080/14786419.2019.1647422. Epub 2019 Jul 30.
Three new acenaphthene derivatives -3-(4'-methoxyphenyl)-acenaphthene-1, 2-diol (), -(1, 2)-3-phenyl-acenaphthene-1, 2-diol () and 8-(4-hydroxyphenyl)-2H-acenaphthylen-1-one () in company with six known compounds were isolated from the 70% ethanol extract of the rhizomes of . Those chemical constituents were separated and purified by macroreticular resin, silica gel, Toyopearl HW-40F, SephadexLH-20 and other chromatographic methods, respectively. The chemical structures of new compounds were elucidated by HR-ESI-MS, H NMR, C NMR, HMQC, HMBC spectrum and specific optical rotations. Compound was isolated for the first time from the genus and compound was firstly assigned the carbon spectrum. Furthermore, the cytotoxic activity of compounds 1-9 against WM9, MDA-MB231, HeLa, K562, DU145 and PC3 was screened with cisplatin as a positive control. Compound showed promising cytotoxic activities with IC values of 2.65 ± 0.38 µM against the HeLa cell lines, while compound possessed significant cytotoxicity with IC values of 6.51 ± 0.44, 18.54 ± 0.68 and 7.98 ± 1.44 µM against the HeLa, MDA-MB231 and WM9 cell lines, respectively.
从 根茎的 70%乙醇提取物中分离得到了三个新的苊烯衍生物-3-(4'-甲氧基苯基)-苊-1,2-二醇()、-(1,2)-3-苯基-苊-1,2-二醇()和 8-(4-羟基苯基)-2H-苊烯-1-酮()，以及六个已知化合物。这些化学成分分别通过大孔树脂、硅胶、ToyopearlHW-40F、SephadexLH-20 等色谱方法进行分离和纯化。通过高分辨电喷雾质谱（HR-ESI-MS）、1H NMR、13C NMR、HMQC、HMBC 谱和比旋光度等方法确定了新化合物的化学结构。化合物 是首次从 属中分离得到的，化合物 是首次对其碳谱进行了归属。此外，还对化合物 1-9 对 WM9、MDA-MB231、HeLa、K562、DU145 和 PC3 的细胞毒性进行了筛选，顺铂为阳性对照。化合物 对 HeLa 细胞系表现出有希望的细胞毒性，IC 值为 2.65±0.38μM，而化合物 对 HeLa、MDA-MB231 和 WM9 细胞系的 IC 值分别为 6.51±0.44、18.54±0.68 和 7.98±1.44μM，表现出显著的细胞毒性。
