Oral and Craniofacial Biomedicine Program, School of Dentistry , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.
Mol Pharm. 2019 Sep 3;16(9):4017-4023. doi: 10.1021/acs.molpharmaceut.9b00671. Epub 2019 Jul 30.
The in vivo antibacterial activity of NO-releasing hyperbranched polymers was evaluated against , a key oral pathogen associated with periodontitis, using a murine subcutaneous chamber model. Escalating doses of NO-releasing polymers (1.5, 7.5, and 37.5 mg/kg) were administered into a -infected chamber once a day for 3 days. Chamber fluids were collected on day 4, with microbiological evaluation indicating a dose-dependent bactericidal action. In particular, NO-releasing polymers at 37.5 mg/kg (1170 μg of NO/kg) achieved complete bacterial eradication (>6-log reduction in bacterial viability), demonstrating greater efficacy than amoxicillin (∼4-log reduction in bacterial viability), a commonly used antibiotic. Time-kill assays further revealed that largest dose (37.5 mg/kg; 1170 μg of NO/kg) resulted in ∼3-log killing of after only a single dose. Based on these results, the potential clinical utility of NO-releasing hyperbranched polymers appears promising, particularly for oral health applications.
体内抗菌活性的 NO 释放超支化聚合物进行了评估，针对 ，一种关键的口腔病原体与牙周炎，用鼠皮下室模型。不断增加的剂量的 NO 释放聚合物 (1.5、7.5 和 37.5 毫克/公斤) 进入一个 - 感染室管理一次一天为 3 天。室液收集第 4 天，与微生物学评价表明剂量依赖性杀菌作用。特别是 NO 释放聚合物在 37.5 毫克/公斤 (1170 μg 的 NO/kg) 达到完全细菌清除 (>6 对数减少细菌的活力)，表现出优于阿莫西林 (∼4 对数减少细菌的活力)，一种常用的抗生素。时间杀伤试验进一步表明，最大剂量 (37.5 毫克/公斤;1170 μg 的 NO/kg) 导致 ∼3 对数杀死 后的单剂量。根据这些结果，NO 释放超支化聚合物的潜在临床应用似乎很有希望，特别是对口腔健康的应用。
