Department of Chemistry, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, United States.
School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States.
Dent Mater. 2020 May;36(5):635-644. doi: 10.1016/j.dental.2020.03.012. Epub 2020 Apr 13.
This study investigates the antibiofilm action of nitric oxide (NO)-releasing hyperbranched polymers against ex vivo multispecies periodontal biofilms.
The antibiofilm efficacy of NO-releasing hyperbranched polymers was evaluated as a function of NO-release properties, polymer concentrations, and oxygen levels in the exposure media. 16s rRNA sequencing technique was employed to evaluate the impact of NO-releasing hyperbranched polymers on the microbial composition of the biofilms.
The addition of NO release significantly improved the antibiofilm action of the hyperbranched polymers, with NO-releasing hyperbranched polyamidoamines of largest NO payloads being more effective than hyperbranched polykanamycins. Furthermore, the NO-releasing hyperbranched polymers reduced the biofilm metabolic activity in a dose-dependent manner, killing biofilm-detached bacteria under both aerobic and anaerobic conditions, with greater antimicrobial efficacy observed under aerobic conditions.
These results demonstrate for the first time the potential therapeutic utility of NO-releasing hyperbranched polymers for treating multispecies dental biofilms.
本研究旨在探讨释放一氧化氮（NO）的超支化聚合物对体外多物种牙周生物膜的抗生物膜作用。
根据 NO 释放特性、聚合物浓度和暴露介质中的氧水平，评估释放 NO 的超支化聚合物的抗生物膜功效。16s rRNA 测序技术用于评估 NO 释放超支化聚合物对生物膜微生物组成的影响。
添加 NO 释放显著提高了超支化聚合物的抗生物膜作用，具有最大 NO 有效载荷的释放 NO 的超支化聚酰胺胺比超支化聚卡那霉素更有效。此外，NO 释放超支化聚合物以剂量依赖性方式降低生物膜代谢活性，在有氧和厌氧条件下均可杀死生物膜游离细菌，有氧条件下观察到更强的抗菌功效。
这些结果首次证明了释放 NO 的超支化聚合物在治疗多物种牙生物膜方面的潜在治疗应用。
