Eldwakhly Elzahraa, Saadaldin Selma, Aldegheishem Alhanoof, Salah Mostafa Marwa, Soliman Mai
Department of Fixed Prosthodontics, Faculty of Dentistry, Cairo University, Cairo 12613, Egypt.
Clinical Dental Science Department, College of Dentistry, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11564, Saudi Arabia.
Pharmaceuticals (Basel). 2020 Oct 29;13(11):350. doi: 10.3390/ph13110350.
This study determined the antimicrobial efficiency of light-activated disinfection (LAD) and photodynamic therapy (PDT) on polymer-infiltrated ceramic network (PICN) material contaminated with three periodontal bacteria and explored if PDT and LAD cause PICN surface alterations. Sixty PICN discs were contaminated with , , and and randomly divided into five groups ( = 12 samples/each) according to the treatment groups: Group PDT-PDT (630 ± 10 nm diode laser) with methylene blue; Group DL-808 nm diode laser in contact mode without photosensitizer; Group MB-methylene blue without light application; Group CHX-0.12% chlorhexidine digluconate solution and; Group NT-no treatment. Each disc was then placed in tubes containing phosphate buffered saline (PBS) and vortexed for 30 s to remove the remaining bacteria from the discs. A total of 10× serial dilutions were performed followed by plating of 30 μL of suspension on Brucella agar plates. The colony forming units (CFU) were calculated after 72 h. PICN discs with the attached biofilms were used for confocal microscopy investigation for live/dead bacterial viability. A random single sample from each group was selected to study the bacterial adherence and topographical alterations on PICN discs under scanning electron microscope (SEM). The PDT group showed higher reduction for each bacterial species and total counts of bacteria assessed followed by the DL group ( < 0.05). When compared with MB group, the two laser groups were significantly superior ( < 0.05). The MB group did not show significant differences for any bacteria when compared to NT. The bacteria with the CHX group and DL groups appeared dead with few areas of surviving green stained bacteria. The PDT group showed the highest dead cell count ( < 0.05). PDT and DL groups indicate no significant changes on the surface compared to the sterile PICN discs on visual assessment. Photodynamic therapy produced superior periodontal bacteria reduction over the surface of PICN surface. PDT group showed higher reduction for each bacterial species and total counts of bacteria assessed followed by the DL group. Both PDT and DL treatment strategies are effective without producing surface alterations on PICN.
本研究测定了光激活消毒（LAD）和光动力疗法（PDT）对被三种牙周细菌污染的聚合物渗透陶瓷网络（PICN）材料的抗菌效率，并探究了PDT和LAD是否会导致PICN表面改变。60个PICN圆盘被 、 和 污染，并根据治疗组随机分为五组（每组 = 12个样本）：PDT组——使用亚甲蓝的630±10 nm二极管激光进行PDT；DL组——808 nm二极管激光接触模式且无光敏剂；MB组——无光照射的亚甲蓝；CHX组——0.12%葡萄糖酸氯己定溶液；NT组——不进行处理。然后将每个圆盘放入含有磷酸盐缓冲盐水（PBS）的试管中，涡旋30秒以去除圆盘上残留的细菌。进行总共10倍系列稀释，随后将30 μL悬浮液接种在布鲁氏菌琼脂平板上。72小时后计算菌落形成单位（CFU）。带有附着生物膜的PICN圆盘用于共聚焦显微镜检查以评估活/死细菌的生存能力。从每组中随机选择一个样本，在扫描电子显微镜（SEM）下研究PICN圆盘上的细菌黏附和形貌改变。PDT组对每种细菌种类和评估的细菌总数显示出更高的减少率，其次是DL组（ < 0.05）。与MB组相比，两个激光组明显更优（ < 0.05）。与NT组相比，MB组对任何细菌均未显示出显著差异。CHX组和DL组的细菌呈现死亡状态，存活的绿色染色细菌区域很少。PDT组显示出最高的死细胞计数（ < 0.05）。与无菌PICN圆盘相比，PDT组和DL组在视觉评估中表面无显著变化。光动力疗法在PICN表面产生了更好的牙周细菌减少效果。PDT组对每种细菌种类和评估的细菌总数显示出更高的减少率，其次是DL组。PDT和DL治疗策略均有效，且不会在PICN上产生表面改变。
