Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Nat Methods. 2019 Aug;16(8):703-706. doi: 10.1038/s41592-019-0498-4. Epub 2019 Jul 29.
Proteins can be phosphorylated at neighboring sites resulting in different functional states, and studying the regulation of these sites has been challenging. Here we present Thesaurus, a search engine that detects and quantifies phosphopeptide positional isomers from parallel reaction monitoring and data-independent acquisition mass spectrometry experiments. We apply Thesaurus to analyze phosphorylation events in the PI3K/AKT signaling pathway and show neighboring sites with distinct regulation.
蛋白质可以在相邻的位点被磷酸化，从而产生不同的功能状态，研究这些位点的调控一直具有挑战性。在这里，我们介绍了 Thesaurus，这是一个搜索引擎，可以从平行反应监测和数据非依赖性采集质谱实验中检测和定量磷酸肽位置异构体。我们将 Thesaurus 应用于分析 PI3K/AKT 信号通路中的磷酸化事件，并展示了具有不同调控的相邻位点。
