School of Life Sciences, Ludong University, Yantai 264025, China.
J Mater Chem B. 2019 Jul 31;7(30):4630-4637. doi: 10.1039/c9tb00845d.
The challenge in antimicrobial photothermal therapy (PTT) is to develop strategies for decreasing the damage to cells and increasing the antibacterial efficiency. Herein, we report a novel theranostic strategy based on bacteria-induced gold nanoparticle (GNP) aggregation, in which GNPs in situ aggregated on the bacterial surface via specific targeting of vancomycin and bioorthogonal cycloaddition. Plasmonic coupling between adjacent GNPs exhibited a strong "hot spot" effect, enabling effective surface enhanced Raman scattering (SERS) imaging of bacterial pathogens. More importantly, in situ aggregation of GNPs showed strong NIR adsorption and high photothermal conversion, allowing enhanced photokilling activity against Gram-positive bacteria. In the absence of bacterial strains, GNPs were dispersed and showed a very low photothermal effect, minimizing the side effects towards surrounding healthy tissues. Given the above advantages, the bioorthogonal theranostic strategy developed in this study may find potential applications in treating bacterial infection and even multidrug-resistant bacteria.
抗菌光热治疗（PTT）面临的挑战是开发降低细胞损伤和提高抗菌效率的策略。在此，我们报告了一种基于细菌诱导的金纳米颗粒（GNP）聚集的新型治疗策略，其中万古霉素的特异性靶向和生物正交环加成使 GNPs 在细菌表面原位聚集。相邻 GNP 之间的等离子体耦合表现出强烈的“热点”效应，能够对细菌病原体进行有效的表面增强拉曼散射（SERS）成像。更重要的是，GNP 的原位聚集表现出强烈的近红外吸收和高光热转换，从而增强了对抗革兰氏阳性菌的光杀伤活性。在没有细菌的情况下，GNPs 是分散的，表现出非常低的光热效应，最大限度地减少了对周围健康组织的副作用。鉴于上述优势，本研究中开发的生物正交治疗策略可能在治疗细菌感染甚至多药耐药菌方面具有潜在的应用。
