University of Belgrade - Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia.
Center for Endocrine Surgery, Clinical Center of Serbia, Dr Subotića 13, Belgrade, Serbia.
Ecotoxicol Environ Saf. 2019 Nov 15;183:109479. doi: 10.1016/j.ecoenv.2019.109479. Epub 2019 Jul 28.
Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.
对组织水平的痕量金属进行评估可以提供比血液或尿液更好的信息，因此可以突出金属在器官特异性疾病病因学中的作用。本研究旨在通过考虑性别、年龄和吸烟习惯，确定健康甲状腺组织 (HTT) 中四种必需 (Mn、Cu、Zn、Se) 和四种有毒金属 (As、Cd、Pb、U) 的基线含量。进一步的目的是检查金属含量是否因良性肿瘤 (BT)、桥本甲状腺炎 (HT)、多结节性甲状腺肿 (MNG) 和甲状腺癌 (TC) 等甲状腺疾病而存在差异。共检测了 423 个组织样本。所有金属均采用电感耦合等离子体质谱法 (ICP-MS) 进行定量分析。结果表明，Cu 和 U 在女性的 HTT 中含量较高，而 Zn 在男性的 HTT 中含量较高。与年轻组 (<50 岁) 相比，50 岁以上 HTT 组的 Zn 含量增加，U 含量降低。Cd、Pb 和 U 的含量增加可将吸烟者与非吸烟者区分开来。与世界其他人群相比，塞尔维亚人群的 Se 含量降低了 15 倍。尽管根据生物变量金属的分布存在差异，但本研究还首次证明，每种甲状腺疾病都有其独特的金属分布。在 HT 组织中发现改变最明显的金属含量。相反，与 HTT 组织最相似的金属含量是 BT 组织。基于含量增加，As、Pb 和 Cd 分别是将 HTT 与 HT、MNG 和 TC 区分开来的主要金属。报告的结果可能突出了有毒和必需痕量金属在甲状腺疾病病因学中尚未得到很好阐明的作用，并且可以为金属对甲状腺健康的有害影响在组织水平上的病理生理变化提供分子基础。
