将三种用于鉴定致病性奈瑟菌属和布兰汉菌属的商业系统与传统方法进行比较评估。

Evaluation of three commercial systems for the identification of pathogenic Neisseria and Branhamella species against the conventional method.

作者信息

Durussel C, Siegrist H H

机构信息

Institut de microbiologie, CHUV, Lausanne, Switzerland.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1988 May;268(3):318-24. doi: 10.1016/s0176-6724(88)80016-6.

DOI:10.1016/s0176-6724(88)80016-6

PMID:3136606

Abstract

We compared three commercial systems for the identification of Neisseria and Branhamella spp. with the conventional method using cystine-tryptic digest agar (CTA) supplemented with carbohydrates, DNase production, and nitrate reduction. We evaluated the API quadFerm+ [( API], Analytab Products, Inc., Plainview, N.Y.), NEISSERIA [( Pasteur], Diagnostics Pasteur, Marnes-la-Coquette, France), and Neisseria Identification Discs [( Oxoid], Oxoid Ltd., Basingstoke, England) using the conventional method as a reference. One hundred and twenty-nine strains were included in this study. The conventional method identified 125 strains (96.9%). Four strains of N. gonorrhoeae remained glucose-negative in the CTA media but gave positive reactions in the API system. API, Pasteur, and Oxoid identified 126 (97.7%), 124 (96.1%), and 62 strains (48.1%), respectively. API and Pasteur seem to be very useful systems for the differentiation of clinically significant species of Neisseria and Branhamella. API has the additional advantage of requiring only a 3 h incubation period.

摘要

我们将三种用于鉴定奈瑟菌属和布兰汉菌属的商业系统与使用补充碳水化合物的胱氨酸胰蛋白酶消化琼脂（CTA）、DNase产生和硝酸盐还原的传统方法进行了比较。我们以传统方法为参考，评估了API quadFerm+ [（API），Analytab Products公司，纽约州普莱恩维尤]、NEISSERIA [（巴斯德），法国巴斯德诊断公司，马恩拉科凯特]和奈瑟菌鉴定盘[（Oxoid），英国奥克托德有限公司，贝辛斯托克]。本研究纳入了129株菌株。传统方法鉴定出125株（96.9%）。4株淋病奈瑟菌在CTA培养基中葡萄糖仍为阴性，但在API系统中呈阳性反应。API、巴斯德和Oxoid分别鉴定出126株（97.7%）、124株（96.1%）和62株（48.1%）。API和巴斯德似乎是区分临床重要奈瑟菌属和布兰汉菌属物种的非常有用的系统。API的另一个优点是只需3小时的孵育期。