Janda W M, Morello J A, Bohnhoff M
J Clin Microbiol. 1984 Mar;19(3):338-41. doi: 10.1128/jcm.19.3.338-341.1984.
The API NeIdent system (Analytab Products, Plainview, N.Y.) was evaluated for identifying Neisseria spp. and Branhamella catarrhalis commonly isolated from clinical specimens. The system identified 90% of 303 Neisseria gonorrhoeae isolates, 71% of 113 Neisseria meningitidis isolates, and 63% of 16 Neisseria lactamica isolates but failed to identify any of 22 B. catarrhalis isolates. Testing of gonococcal strains of various auxotypes revealed no relationship between nutritional requirements and NeIdent profile numbers. With the Neisseria species, interpretation of the cinnamaldehyde-coupled beta-naphthylamine reactions was difficult and resulted in profile numbers not listed in the Profile Register. Positive resazurin-glucose reactions resulted in unlisted numbers for all B. catarrhalis strains. Inconsistent results were also obtained when 62 N. gonorrhoeae isolates were tested more than once on the strip. In all cases, profile variability and failure to identify these organisms were related to the beta-naphthylamide substrate tests. Expansion of the data base and modification of the substrate formulations or their interpretive criteria may increase the reliability of the NeIdent system for identifying Neisseria spp. and B. catarrhalis.
对API NeIdent系统（Analytab Products公司，纽约州普莱恩维尤）进行了评估，以鉴定从临床标本中常见分离出的奈瑟菌属和卡他布兰汉菌。该系统鉴定出303株淋病奈瑟菌中的90%、113株脑膜炎奈瑟菌中的71%以及16株乳酸奈瑟菌中的63%，但未能鉴定出22株卡他布兰汉菌中的任何一株。对各种营养缺陷型的淋球菌菌株进行检测，结果显示营养需求与NeIdent谱号之间没有关系。对于奈瑟菌属，肉桂醛偶联的β-萘胺反应难以解释，导致谱号未列在谱号登记册中。刃天青-葡萄糖阳性反应导致所有卡他布兰汉菌菌株的谱号未列出。当对62株淋病奈瑟菌分离株在试条上进行多次检测时，也得到了不一致的结果。在所有情况下，谱的变异性以及未能鉴定出这些微生物均与β-萘酰胺底物试验有关。扩大数据库以及修改底物配方或其解释标准可能会提高NeIdent系统鉴定奈瑟菌属和卡他布兰汉菌的可靠性。
