Tejano Lhumen A, Peralta Jose P, Yap Encarnacion Emilia S, Chang Yu-Wei
College of Fisheries and Ocean Sciences, Institute of Fish Processing Technology University of the Philippines Visayas Miagao Iloilo Philippines.
Department of Food Science National Taiwan Ocean University Keelung Taiwan.
Food Sci Nutr. 2019 Jun 11;7(7):2381-2390. doi: 10.1002/fsn3.1097. eCollection 2019 Jul.
protein isolates were enzymatically hydrolyzed using pepsin, bromelain, and thermolysin, with their molecular characteristics and bioactivities determined. Thermolysin hydrolysates exhibited the highest degree of hydrolysis (18.08% ± 1.13%). The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results showed that peptides with molecular weights <10 kDa were found in the hydrolysates compared to the protein isolates. Bioactivity assays revealed that pepsin peptide fraction <5 kDa showed the highest angiotensin-converting enzyme (ACE)-inhibitory (34.29% ± 3.45%) and DPPH radical scavenging activities (48.86% ± 1.95%), while pepsin peptide fraction <10 kDa demonstrated the highest reducing power with 0.2101% ± 0.02% absorbance. Moreover, antibacterial assessment revealed that pepsin hydrolysate and peptide fractions displayed inhibition to the test microorganisms. Overall, the present findings suggest that protein hydrolysates can be valuable bio-ingredients with pharmaceutical and nutraceutical application potentials.
使用胃蛋白酶、菠萝蛋白酶和嗜热菌蛋白酶对分离蛋白进行酶解，并测定其分子特性和生物活性。嗜热菌蛋白酶水解产物的水解程度最高（18.08% ± 1.13%）。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结果表明，与分离蛋白相比，水解产物中发现了分子量<10 kDa的肽。生物活性测定表明，<5 kDa的胃蛋白酶肽组分表现出最高的血管紧张素转换酶（ACE）抑制活性（34.29% ± 3.45%）和DPPH自由基清除活性（48.86% ± 1.95%），而<10 kDa的胃蛋白酶肽组分在吸光度为0.2101% ± 0.02%时表现出最高的还原能力。此外，抗菌评估表明，胃蛋白酶水解产物和肽组分对测试微生物有抑制作用。总体而言，目前的研究结果表明，蛋白水解产物可能是具有制药和营养保健应用潜力的有价值生物成分。
