Fu Fanrui, Chauhan Munish, Sadleir Rosalind
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
Biomed Phys Eng Express. 2018 May;4(3). doi: 10.1088/2057-1976/aab72e. Epub 2018 Apr 11.
Spontaneous activity in the abdominal ganglion of can be used as a convenient bioelectricity source in tests of novel MRI-based functional imaging methods, such as functional Magnetic Resonance Electrical Impedance Tomography (fMREIT). In these tests, it is necessary to find a consistent treatment that modulates neural activity, so that these results can be compared with control data. Effects of MREIT imaging currents combined with treatment were also of interest.
Potassium chloride (KCl) was employed as a rhythm modulator. In a series of experiments, effects of adding different volumes of KCl solution were tested and compared with experiments on control groups that had artificial sea water administered. In all cases, neuronal activity was measured with micro electrode arrays.
It was possible to reversibly stop spontaneous activity in ganglia by increasing the extracellular potassium chloride concentration to 89 mM. There was no effect on experimental outcomes when current was administered to the sample chamber between recordings.
KCl can be used as a reversible neural modulator for testing neural detection methods.
在新型基于磁共振成像的功能成像方法（如功能磁共振电阻抗断层成像（fMREIT））的测试中，海兔腹部神经节的自发活动可作为一种便捷的生物电来源。在这些测试中，有必要找到一种能调节神经活动的一致处理方法，以便将这些结果与对照数据进行比较。同时，MREIT成像电流与处理相结合的效果也备受关注。
使用氯化钾（KCl）作为节律调节剂。在一系列实验中，测试添加不同体积KCl溶液的效果，并与给予人工海水的对照组实验进行比较。在所有情况下，均使用微电极阵列测量神经元活动。
通过将细胞外氯化钾浓度提高到89 mM，可以可逆地停止神经节中的自发活动。在记录之间向样品室施加电流时，对实验结果没有影响。
KCl可作为一种可逆的神经调节剂用于测试神经检测方法。
