Robak Paulina, Ożgo Małgorzata, Lepczyński Adam, Herosimczyk Agnieszka, Barszcz Marcin, Taciak Marcin, Skomiał Jacek
Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology Szczecin, Szczecin, Poland.
Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jabłonna, Poland.
J Anim Physiol Anim Nutr (Berl). 2019 Nov;103(6):1837-1847. doi: 10.1111/jpn.13170. Epub 2019 Jul 31.
The aim of the study was to evaluate the influence of dietary supplementation with inulin extract from chicory root and dried chicory root on the protein profile of the renal cortex and medulla of growing pigs. The experiment was carried out on renal cortex and medulla tissue collected from 24 50-day-old PIC x Penarlan P76 crossbred piglets (males). Animals were divided into three dietary groups (n = 8) and fed with a control diet, diet supplemented with 2% inulin extract from chicory root and a diet supplemented with 4% dried chicory root. Kidney samples were collected after 40 days of feeding, and renal cortex and medulla proteins were separated by two-dimensional electrophoresis. Protein identification was performed using MALDI-TOF mass spectrometry. The diet supplemented with 2% chicory inulin induced significant expression changes of 20 and 26 protein spots in the renal cortex and medulla respectively. Supplementation with 4% dried chicory root triggered changes in the expression of 44 and 24 proteins in the renal cortex and medulla respectively. Both forms of chicory inulin-type fructans effectively affected the expression of proteins involved in energy metabolism, heat shock proteins and other chaperones, cytoskeletal and cytoskeleton-related proteins, as well as other proteins. Additionally, changes in transferrin abundance in both experimental groups suggested the significance of chicory fructan supplementation for iron absorption and bioavailability. In conclusion, 2% inulin extract from chicory root and 4% dried chicory root exerted a similar effect on changes in renal protein expression; however, more pronounced alterations were induced by dried chicory root. Nevertheless, further studies are needed for better understanding the mechanism underlying the effect of chicory inulin-type fructans and their fermentation end products on the kidneys of growing pigs.
本研究的目的是评估用菊苣根中提取的菊粉和菊苣根干粉进行日粮补充，对生长猪肾皮质和髓质蛋白质谱的影响。实验采用从24头50日龄的PIC×Penarlan P76杂交仔猪（雄性）采集的肾皮质和髓质组织进行。将动物分为三个日粮组（每组n = 8），分别饲喂对照日粮、添加2%菊苣根中提取菊粉的日粮和添加4%菊苣根干粉的日粮。饲喂40天后采集肾脏样本，通过二维电泳分离肾皮质和髓质蛋白质。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）进行蛋白质鉴定。添加2%菊苣菊粉的日粮分别在肾皮质和髓质中诱导了20个和26个蛋白点的显著表达变化。添加4%菊苣根干粉分别引发了肾皮质和髓质中44个和24个蛋白质表达的变化。两种形式的菊苣菊粉型果聚糖均有效影响了参与能量代谢、热休克蛋白和其他伴侣蛋白、细胞骨架及细胞骨架相关蛋白以及其他蛋白质的表达。此外，两个实验组中转铁蛋白丰度的变化表明菊苣果聚糖补充对铁吸收和生物利用度具有重要意义。总之，2%菊苣根中提取的菊粉和4%菊苣根干粉对肾脏蛋白质表达变化具有相似的影响；然而，菊苣根干粉诱导的变化更为明显。尽管如此，仍需要进一步研究以更好地了解菊苣菊粉型果聚糖及其发酵终产物对生长猪肾脏影响的潜在机制。
