sp. nov.，从日本和缅甸的患者中分离得到。
sp. nov., isolated from patients in Japan and Myanmar.
机构信息
Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
Department of Microbiome Research, Juntendo University School of Medicine, Tokyo, Japan.
出版信息
Int J Syst Evol Microbiol. 2019 Nov;69(11):3377-3384. doi: 10.1099/ijsem.0.003623.
A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, designated as strain BML3, was isolated from a sputum sample of a hospital patient in Japan. Strain BML3 grew at temperatures from 4 to 40 °C, in 1.0-7.0 % (w/v) NaCl and at pH 6.0-9.0. Results of phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA gene and , and , showed that strain BML3 was part of the group and located close to , and . Whole-genome comparisons, using average nucleotide identity and digital DNA-DNA hybridization, confirmed strain BML3 to be a distinct species among the group. Phenotypic characterization tests demonstrated that the utilization of phenylmercuric acetate could distinguish this strain from other closed species of the group. Based on genetic and phenotypic evidence, strain BML3 should be classified as a novel species, for which the name sp. nov. is proposed. The type strain is BML3 (=DSM 109244,=JCM 33395), with a DNA G+C content of 62.66 mol %.
一株革兰氏阴性、需氧、杆状、不产芽孢的细菌，被命名为 BML3 菌株，从日本一位医院患者的痰样中分离得到。BML3 菌株在 4 至 40°C、1.0-7.0%（w/v）NaCl 和 pH 值 6.0-9.0 的条件下生长。基于 16S rRNA 基因和 、 和 等看家基因序列进行的系统发育分析结果表明，BML3 菌株属于 群，与 、 和 关系较近。全基因组比较，使用平均核苷酸同一性和数字 DNA-DNA 杂交，证实 BML3 菌株是 群中一个独特的种。表型特征测试表明，苯汞乙酸盐的利用可以将该菌株与 群中的其他近缘种区分开来。基于遗传和表型证据，BML3 菌株应被分类为一个新种，建议命名为 sp. nov.。其模式菌株是 BML3（=DSM 109244，=JCM 33395），其 DNA G+C 含量为 62.66 mol%。
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