Université Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France.
HCS-Pharma, Rennes Biopôle, 6 rue Pierre Joseph Colin, 35000 Rennes, France.
J Biotechnol. 2019 Sep 10;303:65-71. doi: 10.1016/j.jbiotec.2019.07.010. Epub 2019 Jul 29.
Nowadays, research concerning immunomodulatory products are of great interest, particularly in the treatment of inflammatory diseases or the prevention of infectious diseases. These activities are usually evaluated on cell cultures, by tracking different factors requiring dedicated manipulation. Evaluation of the immunomodulatory activities of essential oils and pure compounds using several technics adapted to high content analysis is described in this study. This approach allows a multiparametric evaluation on a single cell batch, in order to obtain an overall response. The developed method is based on the simultaneous evaluation of phagocytosis, production of iNOS and secretion of IL-6, induced by contact of RAW 264.7 cells with LPS. The results highlight the immunomodulatory activities of cinnamon and clove essential oils. They also provide information, particularly concerning the inhibitory activity of mint essential oil, which inhibits the LPS-induced phagocytosis of RAW 264.7 cells by 42%, at 100 μg/ml. This work presents for the first time the adaptation of high content analyses for the monitoring of immunomodulatory activities of essential oils. This protocol could be adjustable to other cell types and supplemented by the evaluation of additional parameters.
如今，免疫调节产品的研究引起了极大的关注，特别是在治疗炎症性疾病或预防传染病方面。这些活动通常在细胞培养物上进行评估，通过跟踪需要专门操作的不同因素。本研究描述了使用几种适应于高内涵分析的技术评估精油和纯化合物的免疫调节活性。这种方法允许在单个细胞批次上进行多参数评估，以获得整体反应。所开发的方法基于同时评估 RAW 264.7 细胞与 LPS 接触诱导的吞噬作用、iNOS 的产生和 IL-6 的分泌。结果突出了肉桂和丁香精油的免疫调节活性。它们还提供了有关薄荷精油抑制 LPS 诱导的 RAW 264.7 细胞吞噬作用的信息，在 100μg/ml 时抑制率为 42%。这项工作首次提出了适应高内涵分析来监测精油的免疫调节活性。该方案可以调整适用于其他细胞类型，并通过评估其他参数进行补充。
