Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China.
Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China.
Fertil Steril. 2019 Oct;112(4):663-669.e1. doi: 10.1016/j.fertnstert.2019.06.008. Epub 2019 Jul 29.
To evaluate the affect of the duration of donor sperm storage on pregnancy success among women undergoing assisted reproduction.
Retrospective cross-sectional study.
Sperm bank.
PATIENT(S): A total of 119,558 specimens retrieved using a clinical information database of young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank of China from 2001 to 2016.
INTERVENTION(S): Analysis of semen samples and clinical outcomes after semen use.
MAIN OUTCOME MEASURE(S): Clinical information included semen parameters before and after freezing, clinical pregnancy, abortion and live birth rates after semen use.
RESULT(S): The sperm's frozen-thaw survival rate decreased from 85.72% to 73.98% after 15 years of cryopreservation (P<.01). The clinical pregnancy rate of women undergoing artificial insemination with donor sperm was 23.09%, 22.36% and 22.32%, the clinical abortion rate was 10.06%, 10.02% and 12.00% and the live birth rate was 82.17%, 80.21% and 80.00% in the groups with 0.5-5, 6-10 and 11-15 storage years, respectively. The clinical pregnancy rate of women undergoing in vitro fertilization was 64.29%, 64.94% and 53.48%, the clinical abortion rate was 12.26%, 11.38% and 17.39% and the live birth rate was 81.63%, 79.11% and 73.91%, in the groups with 0.5-5, 6-10 and 11-15 years, respectively.
CONCLUSION(S): The long-term cryostorage of semen in a human sperm bank does not affect clinical outcomes. However, cryopreservation longer than 5 years negatively influenced the quality of frozen-thawed donor sperm samples.
评估供精储存时间对接受辅助生殖的女性妊娠成功的影响。
回顾性横断面研究。
精子库。
共 119558 例标本，来源于 2001 年至 2016 年湖南省人类精子库的年轻男性合格供精者的临床信息数据库。
分析精液样本和精液使用后的临床结果。
临床信息包括冻融前和冻融后的精液参数、临床妊娠、流产和活产率。
精子冷冻解冻存活率在 15 年的冷冻保存后从 85.72%下降到 73.98%（P<.01）。接受供精人工授精的女性的临床妊娠率分别为 23.09%、22.36%和 22.32%，临床流产率分别为 10.06%、10.02%和 12.00%，活产率分别为 82.17%、80.21%和 80.00%，储存年限分别为 0.5-5 年、6-10 年和 11-15 年。接受体外受精的女性的临床妊娠率分别为 64.29%、64.94%和 53.48%，临床流产率分别为 12.26%、11.38%和 17.39%，活产率分别为 81.63%、79.11%和 73.91%，储存年限分别为 0.5-5 年、6-10 年和 11-15 年。
人类精子库中精子的长期冷冻储存不会影响临床结局。然而，冷冻保存时间超过 5 年对冷冻解冻供精样本的质量有负面影响。
