蛋黄对结核分枝杆菌在7H12液体培养基中生长的影响。
Effect of egg yolk on growth of Mycobacterium tuberculosis in 7H12 liquid medium.
作者信息
Kononov Y, Ta K D, Heifets L
机构信息
National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
出版信息
J Clin Microbiol. 1988 Jul;26(7):1395-7. doi: 10.1128/jcm.26.7.1395-1397.1988.
Of 92 drug-resistant Mycobacterium tuberculosis strains isolated from sputum specimens, 86 showed growth in two types of 7H12 broth, one with egg yolk and the other without egg yolk. In addition, two strains grew only in plain 7H12 broth without yolk, and four others were recovered only in the medium supplemented with egg yolk. The radiometrically detected growth was higher in the presence of egg yolk, corresponding to a higher number of CFU per milliliter in these cultures. The improvement of growth in 7H12 broth supplemented with egg yolk was most noticeable in cultures isolated from sputum specimens having a low number of acid-fast bacilli in the smear and producing only a few colonies on solid media.
从痰液标本中分离出的92株耐多药结核分枝杆菌菌株中，86株在两种类型的7H12肉汤中生长，一种含有蛋黄，另一种不含蛋黄。此外，两株仅在不含蛋黄的普通7H12肉汤中生长，另外四株仅在添加了蛋黄的培养基中生长。在有蛋黄存在的情况下，放射性检测到的生长更高，这与这些培养物中每毫升更高的菌落形成单位数量相对应。在涂片抗酸杆菌数量少且在固体培养基上仅产生少量菌落的痰液标本分离出的培养物中，添加蛋黄的7H12肉汤中生长的改善最为明显。
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