Romanazzo Sara, Nemec Stephanie, Roohani Iman
Biomaterials Design and Tissue Engineering Lab, School of Chemistry, University of New South Wales, New South Wales 2052, Australia.
School of Materials Science and Engineering, University of New South Wales, New South Wales 2052, Australia.
Materials (Basel). 2019 Aug 1;12(15):2453. doi: 10.3390/ma12152453.
Here, we present a concise review of current 3D bioprinting technologies applied to induced pluripotent stem cells (iPSC). iPSC have recently received a great deal of attention from the scientific and clinical communities for their unique properties, which include abundant adult cell sources, ability to indefinitely self-renew and differentiate into any tissue of the body. Bioprinting of iPSC and iPSC derived cells combined with natural or synthetic biomaterials to fabricate tissue mimicked constructs, has emerged as a technology that might revolutionize regenerative medicine and patient-specific treatment. This review covers the advantages and disadvantages of bioprinting techniques, influence of bioprinting parameters and printing condition on cell viability, and commonly used iPSC sources, and bioinks. A clear distinction is made for bioprinting techniques used for iPSC at their undifferentiated stage or when used as adult stem cells or terminally differentiated cells. This review presents state of the art data obtained from major searching engines, including Pubmed/MEDLINE, Google Scholar, and Scopus, concerning iPSC generation, undifferentiated iPSC, iPSC bioprinting, bioprinting techniques, cartilage, bone, heart, neural tissue, skin, and hepatic tissue cells derived from iPSC.
在此，我们简要综述了当前应用于诱导多能干细胞（iPSC）的3D生物打印技术。iPSC因其独特的特性，最近受到了科学界和临床界的广泛关注，这些特性包括丰富的成体细胞来源、无限自我更新以及分化为身体任何组织的能力。将iPSC及其衍生细胞与天然或合成生物材料相结合进行生物打印，以制造组织模拟构建体，已成为一项可能彻底改变再生医学和个性化治疗的技术。本综述涵盖了生物打印技术的优缺点、生物打印参数和打印条件对细胞活力的影响、常用的iPSC来源以及生物墨水。对于在iPSC未分化阶段或将其用作成体干细胞或终末分化细胞时所使用的生物打印技术进行了明确区分。本综述展示了从主要搜索引擎（包括Pubmed/MEDLINE、谷歌学术和Scopus）获得的关于iPSC生成、未分化iPSC、iPSC生物打印、生物打印技术、软骨、骨、心脏、神经组织、皮肤以及源自iPSC的肝组织细胞的最新数据。
