Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, 56 Ling Yuan Xi Road, Guangzhou, China.
Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, China.
Curr Microbiol. 2019 Nov;76(11):1238-1246. doi: 10.1007/s00284-019-01745-1. Epub 2019 Aug 3.
Streptococcus mutans (S. mutans) adheres to the tooth surface, metabolizes carbohydrates, and produces acid products, leading to enamel demineralization-the onset of dental caries. Rapid acidification by S. mutans has been observed in the presence of glucose. However, little is known about the role of small RNAs (sRNAs) in S. mutans in the presence of glucose and their relationship to tooth adherence. The objective of this study was to evaluate the role of sRNAs in S. mutans (18-50 nucleotides) regarding adherence capacity under 1% and 5% glucose concentrations. The pH drop and adherence capacity in the 1% glucose condition were similar to these parameters under conditions of 5% sucrose that were published in our previous study. A total of 2149 candidate sRNA with at least 100 average reads in the 5% and 1% glucose libraries were obtained. Between the two libraries, 581 sRNAs were differentially expressed and 43 sRNAs were verified. However, the expression levels of the predicted target genes gtfC and spaP were similar between the 1% and 5% glucose conditions. The bioinformatic analysis suggested that differentially expressed sRNAs may be involved in several pathways. These findings indicate that sRNAs were induced under these glucose concentrations and a series of sRNAs were specifically induced, respectively. sRNAs that are induced under glucose stress may be involved in regulating adherence of S. mutans.
变形链球菌（S. mutans）黏附于牙齿表面，代谢碳水化合物，并产生酸产物，导致釉质脱矿——龋齿的发生。在存在葡萄糖的情况下，已经观察到 S. mutans 的快速酸化。然而，关于葡萄糖存在时 sRNAs（18-50 个核苷酸）在 S. mutans 中的作用及其与牙齿黏附的关系知之甚少。本研究旨在评估 sRNAs 在 S. mutans 中关于在 1%和 5%葡萄糖浓度下黏附能力的作用。在 1%葡萄糖条件下的 pH 值下降和黏附能力与我们之前研究中 5%蔗糖条件下的这些参数相似。在 5%和 1%葡萄糖文库中，总共获得了 2149 个平均每个文库至少有 100 个读数的候选 sRNA。在两个文库之间，有 581 个 sRNA 表达差异，有 43 个 sRNA 得到验证。然而，预测靶基因 gtfC 和 spaP 的表达水平在 1%和 5%葡萄糖条件下相似。生物信息学分析表明，差异表达的 sRNA 可能参与了几个途径。这些发现表明，在这些葡萄糖浓度下诱导了 sRNA，并且分别诱导了一系列 sRNA。在葡萄糖应激下诱导的 sRNA 可能参与调节 S. mutans 的黏附。
