乳酸乳球菌亚种双乙酰乳酸亚种α-乙酰乳酸脱羧酶基因在大肠杆菌中的克隆与表达
Cloning and expression of an alpha-acetolactate decarboxylase gene from Streptococcus lactis subsp. diacetylactis in Escherichia coli.
作者信息
Goelling D, Stahl U
机构信息
Technische Universität Berlin, Abteilung Mikrobiologie, Federal Republic of Germany.
出版信息
Appl Environ Microbiol. 1988 Jul;54(7):1889-91. doi: 10.1128/aem.54.7.1889-1891.1988.
Abstract
The Streptococcus lactis gene coding for alpha-acetolactate decarboxylase (ADC) was cloned in Escherichia coli. Subsequent subcloning in E. coli showed that the ADC gene was located within a 1.3-kilobase DNA fragment. The ADC gene was controlled by its own promoter. Gas chromatography showed that S. lactis and the transformed E. coli strains produced the two optical isomers of acetoin in different ratios.
摘要
编码α-乙酰乳酸脱羧酶（ADC）的乳酸链球菌基因在大肠杆菌中被克隆。随后在大肠杆菌中的亚克隆表明，ADC基因位于一个1.3千碱基的DNA片段内。ADC基因由其自身的启动子控制。气相色谱分析表明，乳酸链球菌和转化后的大肠杆菌菌株产生乙偶姻的两种旋光异构体的比例不同。
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