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从脐带血单位获得的纤维蛋白凝胶的短期结果：一项初步体外研究

Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study.

作者信息

Mallis Panagiotis, Gontika Ioanna, Dimou Zetta, Panagouli Effrosyni, Zoidakis Jerome, Makridakis Manousos, Vlahou Antonia, Georgiou Eleni, Gkioka Vasiliki, Stavropoulos-Giokas Catherine, Michalopoulos Efstathios

机构信息

Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece.

Biotechnology division, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece.

出版信息

Bioengineering (Basel). 2019 Aug 2;6(3):66. doi: 10.3390/bioengineering6030066.

DOI:10.3390/bioengineering6030066
PMID:31382445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6783834/
Abstract

BACKGROUND

Recent findings have shown that the fibrin gel derived from cord blood units (CBUs) play a significant role in wound healing and tissue regeneration. The aim of this study was to standardize the fibrin gel production process in order to allow for its regular use.

METHODS

CBUs ( = 200) were assigned to 4 groups according to their initial volume. Then, a two-stage centrifugation protocol was applied in order to obtain platelet rich plasma (PRP). The concentration of platelets (PLTs), white blood cells (WBCs) and red blood cells (RBCs) were determined prior to and after the production process. In addition, targeted proteomic analysis using multiple reaction monitoring was performed. Finally, an appropriate volume of calcium gluconate was used in PRP for the production of fibrin gel.

RESULTS

The results of this study showed that high volume CBUs were characterized by greater recovery rates, concentration and number of PLTs compared to the low volume CBUs. Proteomic analysis revealed the presence of key proteins for regenerative medicine. Fibrin gel was successfully produced from CBUs of all groups.

CONCLUSION

In this study, low volume CBUs could be an alternative source for the production of fibrin gel, which can be used in multiple regenerative medicine approaches.

摘要

背景

最近的研究结果表明，源自脐带血单位（CBUs）的纤维蛋白凝胶在伤口愈合和组织再生中发挥着重要作用。本研究的目的是规范纤维蛋白凝胶的生产过程，以便能够常规使用。

方法

根据初始体积将200个CBUs分为4组。然后，采用两阶段离心方案以获得富含血小板的血浆（PRP）。在生产过程之前和之后测定血小板（PLTs）、白细胞（WBCs）和红细胞（RBCs）的浓度。此外，使用多反应监测进行靶向蛋白质组分析。最后，在PRP中使用适量的葡萄糖酸钙来生产纤维蛋白凝胶。

结果

本研究结果表明，与低体积CBUs相比，高体积CBUs的特点是PLTs的回收率、浓度和数量更高。蛋白质组分析揭示了再生医学关键蛋白的存在。所有组的CBUs均成功生产出纤维蛋白凝胶。

结论

在本研究中，低体积CBUs可能是生产纤维蛋白凝胶的替代来源，该凝胶可用于多种再生医学方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/8e1e6d898d3d/bioengineering-06-00066-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/d28964f20470/bioengineering-06-00066-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/819f8e39e48a/bioengineering-06-00066-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/8e1e6d898d3d/bioengineering-06-00066-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/d28964f20470/bioengineering-06-00066-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/819f8e39e48a/bioengineering-06-00066-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cfd/6783834/8e1e6d898d3d/bioengineering-06-00066-g003.jpg

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