Kerativitayanan Punyavee, Tatullo Marco, Khariton Margarita, Joshi Pooja, Perniconi Barbara, Gaharwar Akhilesh K
Maxillofacial Unit, Calabrodental Clinic, 88900 Crotone, Italy.
Regenerative Medicine Section, Tecnologica Research Institute, 88900 Crotone, Italy.
ACS Biomater Sci Eng. 2017 Apr 10;3(4):590-600. doi: 10.1021/acsbiomaterials.7b00029. Epub 2017 Mar 15.
Synthesis and fabrication of porous and elastomeric nanocomposite scaffolds from biodegradable poly(glycerol sebacate) (PGS) and osteoinductive nanosilicates is reported. Nanosilicates are mineral-based two-dimensional (2D) nanomaterials with high surface area which reinforced PGS network. The addition of nanosilicates to PGS resulted in mechanically stiff and elastomeric nanocomposites. The degradation rate and mechanical stiffness of nanocomposite network could be modulated by addition of nanosilicates. Nanocomposite scaffolds supported cell adhesion, spreading, and proliferation and promoted osteogenic differentiation of preosteoblasts. The addition of nanosilicates to PGS scaffolds increased alkaline phosphatase (ALP) activity and production of matrix mineralization. In vivo studies demonstrated biocompatibility and biodegradability of nanocomposite scaffolds. Overall, the combination of elasticity and tailorable stiffness, tunable degradation profiles, and the osteoinductive capability of the scaffolds offer a promising approach for bone tissue engineering.
报道了由可生物降解的聚癸二酸甘油酯（PGS）和骨诱导性纳米硅酸盐合成及制备多孔弹性体纳米复合支架的方法。纳米硅酸盐是具有高表面积的矿物基二维（2D）纳米材料，可增强PGS网络。向PGS中添加纳米硅酸盐可得到机械刚性和弹性的纳米复合材料。通过添加纳米硅酸盐可以调节纳米复合网络的降解速率和机械刚度。纳米复合支架支持细胞粘附、铺展和增殖，并促进前成骨细胞的成骨分化。向PGS支架中添加纳米硅酸盐可提高碱性磷酸酶（ALP）活性和基质矿化产物。体内研究证明了纳米复合支架的生物相容性和生物降解性。总体而言，支架的弹性和可定制刚度、可调降解特性以及骨诱导能力的结合为骨组织工程提供了一种有前景的方法。
