Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing 312000.
Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing 312000
Biosci Rep. 2019 Aug 23;39(8). doi: 10.1042/BSR20191611. Print 2019 Aug 30.
Breast cancer endangers the life of women and has become the major cause of deaths among them. MiRNAs are found to exert a regulatory effect on the migration, proliferation and apoptosis of breast cancer cells. This research aims at investigating the miR-16-5p expression and its effect on the pathogenesis of breast cancer. Their clinical data were analyzed with qRT-PCR. CCK8, EdU and Transwell was performed to explore the function of miR-16-5p in cell migration and proliferation of breast cancer cells. Dual-luciferase reporter assay, immunohistochemistry and Western blotting were carried out to explore the relation between miR-16-5p and AKT3. It was discovered that miR-16-5p was lowly expressed in breast cancer patients. Meanwhile, breast cancer patients with under-expressed miR-16-5p had a lower survival rate than those with highly expressed miR-16-5p. Furthermore, decreased miR-16-5p in cell and animal models enhanced migration and proliferation of breast cancer cells, stimulated cell cycle and reduced cell apoptosis. Finally, we found miR-16-5p restrained the NF-κB pathway and decreased gene, thereby suppressing the breast cancer development. It can be seen that miR-16-5p exhibits a low expression in breast cancer tissues, which can inhibit breast cancer by restraining the NF-κB pathway and elevating reducing AKT3.
乳腺癌危及女性生命,已成为女性死亡的主要原因。研究发现,miRNAs 对乳腺癌细胞的迁移、增殖和凋亡具有调节作用。本研究旨在探讨 miR-16-5p 的表达及其对乳腺癌发病机制的影响。采用 qRT-PCR 分析其临床数据。CCK8、EdU 和 Transwell 实验探讨 miR-16-5p 对乳腺癌细胞迁移和增殖的功能。双荧光素酶报告实验、免疫组化和 Western blot 实验探讨 miR-16-5p 与 AKT3 之间的关系。结果发现 miR-16-5p 在乳腺癌患者中低表达。同时,miR-16-5p 低表达的乳腺癌患者生存率低于 miR-16-5p 高表达的患者。此外,细胞和动物模型中 miR-16-5p 的减少促进了乳腺癌细胞的迁移和增殖,刺激细胞周期并减少细胞凋亡。最后,我们发现 miR-16-5p 抑制了 NF-κB 通路并降低了基因的表达,从而抑制了乳腺癌的发展。由此可见,miR-16-5p 在乳腺癌组织中表达较低,通过抑制 NF-κB 通路和降低 AKT3 表达来抑制乳腺癌。