Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju, 61186, Republic of Korea.
Antonie Van Leeuwenhoek. 2024 Jul 10;117(1):99. doi: 10.1007/s10482-024-01992-8.
A novel Gram-negative, white-pigmented, and auxin-producing strain, 20NA77.5, was isolated from fresh water during cyanobacterial bloom period. Pairwise comparison of the 16S rRNA gene sequences showed that strain 20NA77.5 belonged to the genus Undibacterium and exhibited the highest sequence similarity to the type strains of Undibacterium danionis (98.00%), Undibacterium baiyunense (97.93%), Undibacterium macrobrachii (97.92%), and Undibacterium fentianense (97.71%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain 20NA77.5 and its related type strains were below 79.93 and 23.80%, respectively. The predominant fatty acids (> 10% of the total fatty acids) were C and summed feature 3 (Cω7c and/or Cω6c). The genomic DNA G + C content of strain 20NA77.5 was found to be 48.61%. Based on the phylogenetic distinctness, chemotaxonomic features, and phenotypic features, strain 20NA77.5 is considered to represent a novel species of the genus Undibacterium, for which the name Undibacterium cyanobacteriorum sp. nov is proposed. The type strain is 20NA77.5 (= KCTC 8005 = LMG 33136).
一株新型革兰氏阴性、白色、产植物生长素的菌株 20NA77.5 是在蓝藻水华期从淡水环境中分离得到的。16S rRNA 基因序列的比对结果显示，菌株 20NA77.5 属于未丁菌属（Undibacterium），与未丁菌属的模式菌株丹氏未丁菌（Undibacterium danionis）（98.00%）、白云未丁菌（Undibacterium baiyunense）（97.93%）、巨形未丁菌（Undibacterium macrobrachii）（97.92%）和奋田未丁菌（Undibacterium fentianense）（97.71%）的相似度最高。菌株 20NA77.5 与相关模式菌株的平均核苷酸一致性（ANI）和数字 DNA-DNA 杂交（dDDH）值均低于 79.93%和 23.80%。其主要脂肪酸（占总脂肪酸的比例＞10%）为 C 和特征脂肪酸 3（Cω7c 和/或 Cω6c）。菌株 20NA77.5 的基因组 DNA G+C 含量为 48.61%。综合系统发育关系、化学分类特征和表型特征，菌株 20NA77.5 代表了未丁菌属的一个新型物种，建议将其命名为 噬藻未丁菌（Undibacterium cyanobacteriorum sp. nov）。其模式菌株为 20NA77.5（=KCTC 8005=LMG 33136）。
