Liu Xiaonan, Zhu Xiaoxi, Wang Hui, Liu Tian, Cheng Jian, Jiang Huifeng
Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Synth Syst Biotechnol. 2020 Jul 1;5(3):187-199. doi: 10.1016/j.synbio.2020.06.008. eCollection 2020 Sep.
Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Over the last few years, there has been remarkable progress in plant P450s identification with the rapid development of sequencing technology, "omics" analysis and synthetic biology. However, challenges still persist in respect of crystal structure, heterologous expression and enzyme engineering. Here, we reviewed several research hotspots of P450 enzymes involved in the biosynthesis of plant natural products, including P450 databases, gene mining, heterologous expression and protein engineering.
细胞色素P450在自然界中广泛存在，在植物天然产物的多样化和功能修饰中发挥关键作用。在过去几年中，随着测序技术、“组学”分析和合成生物学的快速发展，植物P450的鉴定取得了显著进展。然而，在晶体结构、异源表达和酶工程方面仍然存在挑战。在这里，我们综述了参与植物天然产物生物合成的P450酶的几个研究热点，包括P450数据库、基因挖掘、异源表达和蛋白质工程。
