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Trends Microbiol. 2017 Sep;25(9):713-728. doi: 10.1016/j.tim.2017.06.008. Epub 2017 Jul 19.
2
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3
The global burden of respiratory disease.全球呼吸疾病负担。
Ann Am Thorac Soc. 2014 Mar;11(3):404-6. doi: 10.1513/AnnalsATS.201311-405PS.
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Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus.实时逆转录聚合酶链反应检测试剂盒用于中东呼吸综合征冠状病毒。
J Clin Microbiol. 2014 Jan;52(1):67-75. doi: 10.1128/JCM.02533-13. Epub 2013 Oct 23.
5
Field evaluation of TaqMan Array Card (TAC) for the simultaneous detection of multiple respiratory viruses in children with acute respiratory infection.TaqMan 阵列卡(TAC)在儿童急性呼吸道感染中同时检测多种呼吸道病毒的现场评估。
J Clin Virol. 2013 Jul;57(3):254-60. doi: 10.1016/j.jcv.2013.03.016. Epub 2013 Apr 19.
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Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses.快速诊断呼吸道病原体多重实时 RT-PCR 检测与内部单重检测在全面检测人类呼吸道病毒中的比较。
J Virol Methods. 2012 Nov;185(2):259-66. doi: 10.1016/j.jviromet.2012.07.010. Epub 2012 Jul 11.
7
Hospitalization due to human parainfluenza virus-associated lower respiratory tract illness in rural Thailand.泰国农村地区因人类副流感病毒相关下呼吸道疾病而住院的情况。
Influenza Other Respir Viruses. 2013 May;7(3):280-5. doi: 10.1111/j.1750-2659.2012.00393.x. Epub 2012 Jun 21.
8
The burden of hospitalized lower respiratory tract infection due to respiratory syncytial virus in rural Thailand.泰国农村因呼吸道合胞病毒导致的住院下呼吸道感染负担。
PLoS One. 2010 Nov 30;5(11):e15098. doi: 10.1371/journal.pone.0015098.
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Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses.用于全面检测人鼻病毒的实时逆转录-聚合酶链反应检测法
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Human coronavirus infections in rural Thailand: a comprehensive study using real-time reverse-transcription polymerase chain reaction assays.泰国农村地区的人类冠状病毒感染:一项使用实时逆转录聚合酶链反应检测的综合研究。
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通过全球卫生安全议程(GHSA)框架加强检测呼吸道病毒病原体的实验室能力。

Strengthening laboratory capacity for detection of respiratory viral pathogens through the Global Health Security Agenda (GHSA) framework.

作者信息

Whitaker Brett, Alroy Karen A, Guthrie Erica, Schildecker Sarah, Hiers Susan, Woodard Jill, Balajee S Arunmozhi

机构信息

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States.

Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States.

出版信息

Afr J Lab Med. 2019 Jul 18;8(1):861. doi: 10.4102/ajlm.v8i1.861. eCollection 2019.

DOI:10.4102/ajlm.v8i1.861
PMID:31392168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6676779/
Abstract

BACKGROUND

Endemic and emerging respiratory viruses are a threat to public health, and a robust public health laboratory system is essential to ensure global health security.

OBJECTIVE

This program sought to expand molecular laboratory testing capacity to detect a broad range of respiratory pathogens in clinical respiratory specimens collected during disease surveillance and outbreak investigations.

METHODS

As a part of the Global Health Security Agenda (GHSA), the United States Centers for Disease Control and Prevention utilised the equipment and training infrastructure already in place at the World Health Organization National Influenza Centers to expand testing capacity for respiratory viruses in laboratories in GHSA partner countries. This was done through the provision of quality assured reagents, including multiplex platforms and technical guidance for laboratory staff, as well as the assessment of laboratory testing accuracy.

CONCLUSION

Early findings illustrated that GHSA laboratories have been able to expand testing capacity using specimens from routine surveillance, as well as from outbreak situations.

摘要

背景

地方性和新出现的呼吸道病毒对公众健康构成威胁,强大的公共卫生实验室系统对于确保全球卫生安全至关重要。

目的

该项目旨在扩大分子实验室检测能力,以检测在疾病监测和疫情调查期间收集的临床呼吸道标本中的多种呼吸道病原体。

方法

作为全球卫生安全议程(GHSA)的一部分,美国疾病控制与预防中心利用世界卫生组织国家流感中心现有的设备和培训基础设施,扩大GHSA伙伴国家实验室对呼吸道病毒的检测能力。这是通过提供质量有保证的试剂(包括多重检测平台)、为实验室工作人员提供技术指导以及评估实验室检测准确性来实现的。

结论

早期结果表明,GHSA实验室已能够利用常规监测标本以及疫情情况下的标本扩大检测能力。