Nishikawa S I, Hirata T, Izumi T
J Immunol. 1979 Sep;123(3):1106-9.
We investigated the relationship bewteen PPD-reactive helper T cells and PWM-reactive helper T cells in polyclonal Ig production of human PBL. Elimination of PPD-reactive T cells by BUdR + light treatment resulted in a loss of helper function in PPD-induced Ig production, but had no effect on helper function in PWM-induced Ig production. On the other hand, elimination of PWM-reactive T cells resulted in a loss of helper function in both PPD-induced Ig production and PWM-induced Ig production. A blast cell-enriched fraction that was generated by PPD and separated by the velocity sedimentation method contained helper function in both responses. On the other hand, blast cell-depleted fractions did not contain PPD-reactive helper function, although the PWM-reactive helper function was evident. These results strongly suggest that PPD-reactive helper T cells are included in PWM-reactive helper T cells.
我们研究了人外周血淋巴细胞（PBL）多克隆免疫球蛋白（Ig）产生过程中，PPD反应性辅助性T细胞与PWM反应性辅助性T细胞之间的关系。通过溴脱氧尿苷（BUdR）+光照处理消除PPD反应性T细胞，导致PPD诱导的Ig产生中辅助功能丧失，但对PWM诱导的Ig产生中的辅助功能没有影响。另一方面，消除PWM反应性T细胞导致PPD诱导的Ig产生和PWM诱导的Ig产生中的辅助功能均丧失。由PPD产生并通过速度沉降法分离的富含母细胞的部分在两种反应中均具有辅助功能。另一方面，尽管PWM反应性辅助功能明显，但去除母细胞的部分不包含PPD反应性辅助功能。这些结果有力地表明，PPD反应性辅助性T细胞包含在PWM反应性辅助性T细胞中。
