Nishikawa S I, Hirata T, Nagai T, Mayumi M, Izumi T
J Immunol. 1979 Jun;122(6):2143-9.
The ability of PPD to induce Ig production in human PBL was investigated. PPD proved to be a good B cell activator for inducing polyclonal Ig production in PBL from healthy Japanese. Comparative studies of this response with PWM-induced Ig production showed that the cellular mechanisms involved in the two responses were different. First, PBL from an atypical individual with a deficient IgM production to PWM responded normally to PPD with IgM production as well as IgG production. Secondly, in IgG production, the effects of the two mitogens (PPDand PWM) were additive. An analysis of the cellular requirements in PPD-induced Ig production clearly demonstrated that T cells played a role in this response as well as in the PWM-induced response. However, the head-to-head comparative study on the titration curves of helper T cells in the two responses showed that PWM-induced helper activity was 2 to 5 times more effective than PPD-induced helper activity. Moreover, PPD-induced helper activity was shown to be more sensitive to ionizing radiation than was PWM-induced helper activity. Thus, this system of PPD-induced Ig production may provide a useful tool for understanding the human antibody production system as well as the PWM-induced response.
研究了结核菌素纯蛋白衍生物（PPD）诱导人外周血淋巴细胞（PBL）产生免疫球蛋白（Ig）的能力。结果证明，PPD是一种良好的B细胞激活剂，可诱导健康日本人的PBL产生多克隆Ig。对该反应与美洲商陆有丝分裂原（PWM）诱导的Ig产生进行比较研究表明，两种反应所涉及的细胞机制不同。首先，对PWM产生IgM能力缺陷的非典型个体的PBL对PPD的反应正常，可产生IgM和IgG。其次，在IgG产生方面，两种有丝分裂原（PPD和PWM）的作用是相加的。对PPD诱导的Ig产生中细胞需求的分析清楚地表明，T细胞在该反应以及PWM诱导的反应中均发挥作用。然而，对两种反应中辅助性T细胞滴定曲线的直接比较研究表明，PWM诱导的辅助活性比PPD诱导的辅助活性高2至5倍。此外，PPD诱导的辅助活性比PWM诱导的辅助活性对电离辐射更敏感。因此，PPD诱导的Ig产生系统可能为理解人类抗体产生系统以及PWM诱导的反应提供一个有用的工具。
