He Mengshan, Long Pan, Guo Lunfeng, Zhang Mingke, Wang Siwang, He Hongling
Department of Chinese Material Medical and Natural Medicines, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Center of Clinical Aerospace Medicine, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Evid Based Complement Alternat Med. 2019 Jul 18;2019:5376439. doi: 10.1155/2019/5376439. eCollection 2019.
Diabetic retinopathy (DR) remains one of the leading causes of acquired blindness. Fushiming capsule (FSM), a compound traditional Chinese medicine, is clinically used for DR treatment in China. The present study was to investigate the effect of FSM on retinal alterations, inflammatory response, and oxidative stress triggered by diabetes.
Diabetic rat model was induced by 6-week high-fat and high-sugar diet combined with 35 mg/kg streptozotocin (STZ). 30 days after successful establishment of diabetic rat model, full field electroretinography (ffERG) and optical coherence tomography (OCT) were performed to detect retinal pathological alterations. Then, FSM was administered to diabetic rats at different dosages for 42-day treatment and diabetic rats treated with Calcium dobesilate (CaD) capsule served as the positive group. Retinal function and structure were observed, and retinal vascular endothelial growth factor- (VEGF-), glial fibrillary acidic (GFAP), and vascular cell adhesion protein-1 (VCAM-1) expressions were measured both on mRNA and protein levels, and a series of blood metabolic indicators were also assessed.
In DR rats, FSM (1.0g/kg and 0.5g/kg) treatment significantly restored retinal function (a higher amplitude of b-wave in dark-adaptation 3.0 and OPs2 wave) and prevented the decrease of retinal thickness including inner nuclear layer (INL), outer nuclear layer (ONL), and entire retina. Additionally, FSM dramatically decreased VEGF-, GFAP, and VCAM-1 expressions in retinal tissues. Moreover, FSM notably improved serum antioxidative enzymes glutathione peroxidase, superoxide dismutase, and catalase activities, whereas it reduced serum advanced glycation end products, methane dicarboxylic aldehyde, nitric oxide, and total cholesterol and triglycerides levels.
FSM could ameliorate diabetic rat retina damage possibly via inhibiting inflammation and improving antioxidation.
糖尿病视网膜病变（DR）仍是后天性失明的主要原因之一。复明胶囊（FSM）是一种复方中药，在中国临床上用于治疗DR。本研究旨在探讨FSM对糖尿病引发的视网膜病变、炎症反应及氧化应激的影响。
采用6周高脂高糖饮食联合35mg/kg链脲佐菌素（STZ）诱导糖尿病大鼠模型。糖尿病大鼠模型成功建立30天后，进行全视野视网膜电图（ffERG）和光学相干断层扫描（OCT）检测视网膜病理改变。然后，给予不同剂量的FSM对糖尿病大鼠进行42天治疗，以羟苯磺酸钙（CaD）胶囊治疗的糖尿病大鼠作为阳性对照组。观察视网膜功能和结构，并检测视网膜血管内皮生长因子（VEGF）、胶质纤维酸性蛋白（GFAP）和血管细胞黏附蛋白1（VCAM-1）在mRNA和蛋白水平的表达，同时评估一系列血液代谢指标。
在DR大鼠中，FSM（1.0g/kg和0.5g/kg）治疗显著恢复了视网膜功能（暗适应3.0时b波和OPs2波振幅更高），并防止了视网膜厚度的降低，包括内核层（INL）、外核层（ONL）和整个视网膜。此外，FSM显著降低了视网膜组织中VEGF、GFAP和VCAM-1的表达。而且，FSM显著提高了血清抗氧化酶谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性，同时降低了血清晚期糖基化终产物、甲基乙二醛、一氧化氮以及总胆固醇和甘油三酯水平。
FSM可能通过抑制炎症和改善抗氧化作用来减轻糖尿病大鼠视网膜损伤。
