1Department of Microbiology & Immunology, University of Minnesota, 689 23rd Avenue SE, Minneapolis, MN 55455 USA.
2Present Address: Department of Genome Sciences, University of Washington, 3720 15th Ave NE, Seattle, WA 98195 USA.
NPJ Biofilms Microbiomes. 2019 Aug 6;5(1):20. doi: 10.1038/s41522-019-0093-6. eCollection 2019.
has attracted increasing attention as an emerging pathogen in patients with cystic fibrosis. Intrinsic resistance to several classes of antimicrobials and the ability to form robust biofilms in vivo contribute to the clinical manifestations of persistent infection. Still, much of biofilm formation remains uncharacterized due to the scarcity of existing genetic tools. Here we demonstrate a promising genetic system for use in ; generating a transposon mutant library which was then used to identify genes involved in biofilm development in vitro. We further described the effects of one of the genes found in the mutagenesis screen, encoding a putative enoyl-CoA hydratase, on biofilm structure and tolerance to antimicrobials. Through additional analysis, we find that a fatty acid signaling compound is essential to biofilm ultrastructure and maintenance. This work describes methods for the genetic manipulation of and demonstrated their use to improve our understanding of pathophysiology.
已作为囊性纤维化患者的一种新兴病原体而受到越来越多的关注。对几类抗生素的固有耐药性以及在体内形成坚固生物膜的能力导致了持续感染的临床表现。然而，由于现有的遗传工具稀缺，生物膜形成的大部分仍然不明确。在这里，我们展示了一种有前途的遗传系统，用于生成转座子突变体文库，然后该文库用于鉴定体外生物膜发育中涉及的基因。我们进一步描述了诱变筛选中发现的一个基因（编码假定的烯酰辅酶 A 水合酶）对生物膜结构和对抗生素的耐受性的影响。通过进一步分析，我们发现脂肪酸信号化合物对生物膜超微结构和维持至关重要。这项工作描述了 的遗传操作方法，并证明它们可用于提高我们对 发病机制的理解。
