State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China and National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
Food Funct. 2019 Sep 1;10(9):5398-5412. doi: 10.1039/c9fo00278b. Epub 2019 Aug 9.
In this study, the effects of three doses (diets containing <0.01, 0.15, 0.40 mg kg Se) and two forms (sodium selenite and selenomethionine) of dietary Se supplementation on the intestinal physiology of untreated, dextran sodium sulfate-treated, and Salmonella typhimurium-infected mice were evaluated. The underlying modes of action of the varied doses and forms of Se supplementation were analyzed using fecal metabolomic and jejunal proteomic approaches. Compared with adequate Se (0.15 mg kg Se) supplementation, Se-deficiency supplementation adversely affected the gut barrier and intestinal immune responses of the untreated mice and increased their susceptibility to experimental colitis and pathogen infection. In contrast, supranutritional Se (0.40 mg kg Se) supplementation improved mouse intestinal physiology compared with adequate Se supplementation. Varied doses of Se supplementation differentially perturbed the fecal metabolic profiles of and jejunal protein expression in mice. Further, both forms of dietary Se supplementation, i.e., sodium selenite and selenomethionine, showed similar effects on the gut barrier and intestinal immune homeostasis but differentially affected fecal metabolites, such as neurosubstances and immunomodulators, and induced significant proteomic variations in various pathways, including the xenobiotic detoxification pathway and glutathione metabolism. Our results indicate that the doses and chemical forms of Se should be considered when developing dietary nutritional supplements for gut health.
本研究评估了三种硒（膳食中硒含量分别为<0.01、0.15 和 0.40mg/kg）和两种硒形式（亚硒酸钠和硒代蛋氨酸）对未处理、葡聚糖硫酸钠处理和鼠伤寒沙门氏菌感染的小鼠肠道生理学的影响。使用粪便代谢组学和空肠蛋白质组学方法分析了不同剂量和硒补充形式的潜在作用机制。与适量硒（0.15mg/kg 硒）补充相比，硒缺乏补充会对未处理的小鼠肠道屏障和肠道免疫反应产生不利影响，增加其对实验性结肠炎和病原体感染的易感性。相比之下，超营养硒（0.40mg/kg 硒）补充比适量硒补充更能改善小鼠的肠道生理学。硒补充的不同剂量会使小鼠粪便代谢谱和空肠蛋白表达产生差异。此外，两种形式的膳食硒补充剂，即亚硒酸钠和硒代蛋氨酸，对肠道屏障和肠道免疫稳态有相似的影响，但对粪便代谢物（如神经递质和免疫调节剂）有不同的影响，并在包括外源物质解毒途径和谷胱甘肽代谢在内的各种途径中诱导显著的蛋白质组学变化。我们的研究结果表明，在开发用于肠道健康的膳食营养补充剂时，应考虑硒的剂量和化学形式。
