Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, Auf der Morgenstelle 28, D-72076, Tübingen, Germany.
Institute for Molecular Infection Biology (IMIB), University of Würzburg, Josef-Schneider-Strasse 2, D-97080, Würzburg, Germany.
Nat Commun. 2019 Aug 9;10(1):3627. doi: 10.1038/s41467-019-11547-5.
The mechanisms behind carbon dioxide (CO) dependency in non-autotrophic bacterial isolates are unclear. Here we show that the Staphylococcus aureus mpsAB operon, known to play a role in membrane potential generation, is crucial for growth at atmospheric CO levels. The genes mpsAB can complement an Escherichia coli carbonic anhydrase (CA) mutant, and CA from E. coli can complement the S. aureus delta-mpsABC mutant. In comparison with the wild type, S. aureus mps mutants produce less hemolytic toxin and are less virulent in animal models of infection. Homologs of mpsA and mpsB are widespread among bacteria and are often found adjacent to each other on the genome. We propose that MpsAB represents a dissolved inorganic carbon transporter, or bicarbonate concentrating system, possibly acting as a sodium bicarbonate cotransporter.
二氧化碳（CO）依赖性在非自养细菌分离物中的作用机制尚不清楚。在这里，我们表明金黄色葡萄球菌 mpsAB 操纵子，已知在膜电位产生中发挥作用，对于在大气 CO 水平下的生长至关重要。mpsAB 基因可以补充大肠杆菌碳酸酐酶（CA）突变体，而大肠杆菌的 CA 可以补充金黄色葡萄球菌 delta-mpsABC 突变体。与野生型相比，金黄色葡萄球菌 mps 突变体产生的溶血毒素较少，在感染动物模型中的毒力较低。mpsA 和 mpsB 的同源物在细菌中广泛存在，并且通常在基因组上彼此相邻。我们提出 MpsAB 代表一种溶解无机碳转运体或碳酸氢盐浓缩系统，可能作为一种碳酸氢钠共转运体。
