Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, Murcia, Spain.
Clin Oral Investig. 2020 May;24(5):1749-1759. doi: 10.1007/s00784-019-03036-2. Epub 2019 Aug 9.
Compositional modifications may alter the biological and physicochemical characteristics of calcium silicate-based sealers (CSBS) and, ultimately, their bioactivity. The main objective of this study was to evaluate the biological properties of three CSBS: EndoSequence BC Sealer, Ceraseal, and Endoseal mineral trioxide aggregate.
Human periodontal ligament stem cells (hPDLSCs) were exposed to several eluates of CSBS. The ion release profile and pH were determined, and metabolic activity and cell migration were assessed using the MTT and wound healing assays. hPDLSCs were cultured in direct contact with the surface of each material, and cell morphology and attachment were analyzed by scanning electron microscopy (SEM). Bioactivity potential was assessed by RT-qPCR and mineralization assays. Statistical differences between biomaterials were assessed using one- or two-way ANOVA (α < 0.05).
All materials showed an alkaline pH, although Endoseal exhibited a significantly higher pH compared with the other CSBS (p < 0.05). Ceraseal released significantly more Ca (p < 0.05) than EndoSequence BC Sealer and Endoseal. Interestingly, Endoseal induced a significant reduction in cell viability and cell migration compared with the control (p < 0.001). Moreover, SEM showed abundant cells adhering to EndoSequence BC Sealer and Ceraseal surfaces, whereas very few round cells were detected on the surface of Endoseal. Finally, Ceraseal and EndoSequence induced ALP, CAP, and CEMP-1 expression and a significantly higher mineralization capacity than Endoseal (***p < 0.001).
The eluates from EndoSequence BC Sealer and Ceraseal displayed higher cell viability, cell attachment, cell migration rates, and ion release rates than Endoseal. Ceraseal and EndoSequence BC Sealer exhibited significantly more gene expression and mineralization capacity than Endoseal.
The results obtained in the present work suggest that EndoSequence BC Sealer and Ceraseal possess biological properties that make them suitable materials for root canal treatment.
组成修饰可能改变基于硅酸钙的密封剂（CSBS）的生物学和物理化学特性，并最终改变其生物活性。本研究的主要目的是评估三种 CSBS 的生物学特性：EndoSequence BC 密封剂、Ceraseal 和 Endoseal 矿化三氧化物聚合体。
将人牙周膜干细胞（hPDLSCs）暴露于几种 CSBS 的洗脱液中。测定离子释放曲线和 pH 值，并通过 MTT 和划痕愈合试验评估代谢活性和细胞迁移。hPDLSCs 与每种材料的表面直接接触培养，通过扫描电子显微镜（SEM）分析细胞形态和附着。通过 RT-qPCR 和矿化试验评估生物活性潜能。使用单因素或双因素方差分析（α<0.05）评估生物材料之间的统计学差异。
所有材料均呈碱性 pH 值，尽管 Endoseal 的 pH 值明显高于其他 CSBS（p<0.05）。Ceraseal 释放的 Ca 明显多于 EndoSequence BC Sealer 和 Endoseal（p<0.05）。有趣的是，与对照组相比，Endoseal 显著降低了细胞活力和细胞迁移（p<0.001）。此外，SEM 显示大量细胞附着在 EndoSequence BC Sealer 和 Ceraseal 表面，而在 Endoseal 表面检测到很少的圆形细胞。最后，Ceraseal 和 EndoSequence 诱导 ALP、CAP 和 CEMP-1 表达，并具有比 Endoseal 更高的矿化能力（***p<0.001）。
与 Endoseal 相比，EndoSequence BC Sealer 和 Ceraseal 的洗脱液具有更高的细胞活力、细胞附着、细胞迁移率和离子释放率。Ceraseal 和 EndoSequence BC Sealer 的基因表达和矿化能力明显高于 Endoseal。
本工作获得的结果表明，EndoSequence BC Sealer 和 Ceraseal 具有生物学特性，使其成为根管治疗的合适材料。
