Department of Stomatology, Faculty of Medicine and Dentistry, Universitat de València, 46010, Valencia, Spain.
Cellular Therapy and Hematopoietic Transplant Research Group, Biomedical Research Institute, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, 30120, Murcia, Spain.
Clin Oral Investig. 2021 Mar;25(3):1451-1462. doi: 10.1007/s00784-020-03453-8. Epub 2020 Jul 10.
The aim of this study was to evaluate the microstructural composition, ion release, cytocompatibility, and mineralization potential of Bio-C Sealer ION+ (BCI) and EndoSequence BC Sealer HiFlow (BCHiF), compared with AH Plus (AHP), in contact with human periodontal ligament cells (hPDLCs).
The sealers' ionic composition and release were assessed using energy-dispersive spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. For the biological assays, hPDLCs were isolated from third molars, and sealer extracts were prepared (undiluted, 1:2, and 1:4 ratios). An MTT assay, wound-healing assay, and cell morphology and adhesion analysis were performed. Activity-related gene expression was determined using RT-qPCR, and mineralization potential was assessed using Alizarin Red staining (ARS). Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test (α < 0.05).
The three sealers exhibited variable levels of silicon, calcium, zirconium, and tungsten release and in their composition. Both BCI and BCHiF groups showed positive results in cytocompatibility assays, unlike AHP. The BCHiF group showed an upregulation of CAP (p < 0.01), CEMP1, ALP, and RUNX2 (p < 0.001) compared with the negative control, while the BCI group showed an upregulation of CEMP1 (p < 0.01), CAP, and RUNX2 (p < 0.001). Both groups also exhibited a greater mineralization potential than the negative and positive controls (p < 0.001).
The calcium silicate-based sealers considered in the present in vitro study exhibited a high calcium ion release, adequate cytocompatibility, upregulated osteo/cementogenic gene expression, and increased mineralized nodule formation in contact with hPDLCs.
From a biological perspective, BCI and BCHiF could be clinically suitable for root canal filling.
本研究旨在评估 Bio-C Sealer ION+（BCI）和 EndoSequence BC Sealer HiFlow（BCHiF）与 AH Plus（AHP）相比，与人类牙周膜细胞（hPDLCs）接触时的微观结构组成、离子释放、细胞相容性和矿化潜力。
使用能量色散光谱（EDS）和电感耦合等离子体质谱（ICP-MS）分别评估密封剂的离子组成和释放。对于生物测定，从第三磨牙中分离 hPDLCs，并制备密封剂提取物（未稀释、1:2 和 1:4 比例）。进行 MTT 测定、划痕愈合测定以及细胞形态和黏附分析。使用 RT-qPCR 测定活性相关基因表达，并用茜素红染色（ARS）评估矿化潜力。使用单向方差分析和 Tukey 事后检验（α<0.05）进行统计分析。
三种密封剂在其组成和硅、钙、锆和钨的释放水平上表现出不同的特性。BCI 和 BCHiF 组的细胞相容性测定结果均为阳性，而 AHP 组则为阴性。与阴性对照组相比，BCHiF 组的 CAP（p<0.01）、CEMP1、ALP 和 RUNX2 表达上调（p<0.001），而 BCI 组的 CEMP1（p<0.01）、CAP 和 RUNX2 表达上调（p<0.001）。与阴性和阳性对照组相比，两组的矿化潜力也更大（p<0.001）。
在本体外研究中，所考虑的基于硅酸钙的密封剂具有较高的钙离子释放、足够的细胞相容性、上调的成骨/成牙骨质基因表达以及与 hPDLCs 接触时增加的矿化结节形成。
从生物学角度来看，BCI 和 BCHiF 可能在临床根管填充方面具有适用性。
