Department of Pharmaceutical Technology, College of Pharmacy, Rangsit University, Pathumthani, 12000, Thailand.
Cosmeceutical Research, Development and Testing Center, College of Pharmacy, Rangsit University, Pathumthani, 12000, Thailand.
Int J Cosmet Sci. 2019 Dec;41(6):548-557. doi: 10.1111/ics.12571.
Grammatophyllum speciosum is utilized to treat sore throats and bronchitis In Thai folk medicine. This study evaluated the in vitro activity and clinical efficacy of a G. speciosum pseudobulb decoction.
Measure of in vitro anti-ageing activity was performed using non-cell based assays as well as in CRL 2097 human fibroblast cells. A prophetic patch test method was used to determine skin irritation in 24 healthy Thai volunteers. A randomized double-blind, placebo-controlled trial was conducted with 24 subjects for 56 days after facial application to evaluate efficacy. The results were measured with Visioface and Cutometer MPA 580 as well as by visual observations.
The total content of the antioxidant polyphenols in G. speciosum ethanolic extract (GSE) was 48.19 ± 0.39 mg EGCG equivalent per gram. The GSE possessed potent and higher anti-elastase activity more than EGCG. The extract was able to protect human fibroblasts against superoxide anion-induced cell death at the concentration of 10 µg mL . In a clinical study, facial application of the serum containing 0.5% GSE was found to safely increase skin distensibility in healthy volunteers. Skin viscoelasticity and wrinkle volume were also significantly reduced (P < 0.05).
Thus, both the in vitro and the clinical studies have illustrated the anti-wrinkle/anti-ageing benefits of GSE on human skin.
泰国民间医学用球兰治疗喉咙痛和支气管炎。本研究评估了球兰假鳞茎汤剂的体外活性和临床疗效。
使用非细胞基础测定法和 CRL 2097 人成纤维细胞测定体外抗老化活性。采用预测斑贴试验法在 24 名健康泰国志愿者中测定皮肤刺激性。对 24 名受试者进行了为期 56 天的面部应用随机双盲、安慰剂对照试验，以评估疗效。结果通过 Visioface 和 Cutometer MPA 580 以及视觉观察进行测量。
球兰乙醇提取物（GSE）中总抗氧化多酚含量为 48.19 ± 0.39 mg EGCG 当量/克。GSE 具有强大的、高于 EGCG 的抗弹性蛋白酶活性。该提取物能够在 10 μg mL 的浓度下保护人成纤维细胞免受超氧阴离子诱导的细胞死亡。在一项临床研究中，发现含有 0.5%GSE 的血清对面部应用能够安全地增加健康志愿者的皮肤延展性。皮肤粘弹性和皱纹体积也显著减少（P<0.05）。
因此，体外和临床研究都说明了 GSE 对人体皮肤的抗皱/抗衰老益处。
