Cosmeceutical Research, Development and Testing Center, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, Thailand.
Department of Pharmaceutical Technology, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, Thailand.
ScientificWorldJournal. 2021 Oct 4;2021:3752169. doi: 10.1155/2021/3752169. eCollection 2021.
/. Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. . The antiproliferation effects of the pseudobulb ethanolic extract (GSE) and isovitexin (bioactive constituent) were investigated on the MCF-7 human breast cancer cell line using MTT and colony formation assay. The expression levels of proliferation-regulatory proteins were determined by western blotting. . Noncytotoxic concentrations of GSE significantly suppressed the proliferation of MCF-7 cells. Tumor colony formation decreased in both number and size. The level of phosphorylated AKT and -catenin was suppressed by GSE treatment. Antiproliferation was observed in isovitexin-treated MCF-7 cells in the form of inhibited colony formation and reduced expression of phosphorylated AKT and -catenin protein. . This study demonstrates the novel effect of as an antiproliferative via suppression of the AKT/-catenin-dependent pathway. This may prompt further investigation of this plant in breast cancer therapy.
/. Blume 表现出多种有前途的药理活性。然而，其对乳腺癌的作用尚未确定。. 采用 MTT 和集落形成实验，研究了假鳞茎乙醇提取物（GSE）和异荭草素（生物活性成分）对 MCF-7 人乳腺癌细胞系的增殖抑制作用。通过蛋白质印迹法测定增殖调节蛋白的表达水平。. GSE 的非细胞毒性浓度显著抑制 MCF-7 细胞的增殖。肿瘤集落的数量和大小均减少。GSE 处理后 AKT 和 -catenin 的磷酸化水平受到抑制。异荭草素处理的 MCF-7 细胞表现出增殖抑制，磷酸化 AKT 和 -catenin 蛋白的表达减少。. 本研究表明 作为一种通过抑制 AKT/-catenin 依赖性途径的增殖抑制剂的新作用。这可能促使进一步研究这种植物在乳腺癌治疗中的应用。
