Zhang Bing, Nurland Rewuzi Aguli, Guan Yaqun, Zhou Shuangshuang, Lu Miao, Nuli Rebiya, Gao Fan, Wang Xiao, Li Kun
Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases, School of Basic Medical Sciences Xinjiang Medical University, 830011, Urumqi City, China.
Xinjiang 474 Hospital, 830000, Urumqi City, China.
New Microbes New Infect. 2023 Sep 1;54:101170. doi: 10.1016/j.nmni.2023.101170. eCollection 2023 Sep.
The blood-feeding behavior of kissing bugs (subfamily Triatominae, family Reduviidae, order Hemiptera) means they are potential vectors of multiple humans pathogens. However, investigations of vector-borne pathogens harbored by kissing bugs are rare.
In the current study, 22 adult kissing bugs () were captured in Huizhou City, Guangdong Province, south China. The presence of vector-borne pathogens in the kissing bugs was tested, and the genetic diversity of these potential pathogens was investigated.
All the kissing bugs were negative for Anaplasmataceae bacteria, , and . DNA was detected in 36.4% (8/22) of the kissing bugs. The sequences of the genes divided into two clades in a phylogenetic tree, with close relationships to and uncultured sp. clone MYR-283, respectively. All the sequences were closely related to those of (identity 98.75%-100%). The and sequences were most closely related to those of , a recognized human pathogen, with nucleotide similarities of 98.70%-100% and 99.45%-100%, respectively.
We report the detection of DNA in kissing bugs in southern China. Although the sample size is limited, the high positive rate of detection of DNA, the close relationship of the gene sequences to those of zoonotic species, and the distribution of the kissing bugs near human residences, hint at a risk to public health.
锥蝽（半翅目猎蝽科锥蝽亚科）的吸血行为意味着它们是多种人类病原体的潜在传播媒介。然而，对锥蝽携带的媒介传播病原体的调查却很少见。
在本研究中，在中国南方广东省惠州市捕获了22只成年锥蝽。检测锥蝽中媒介传播病原体的存在情况，并调查这些潜在病原体的遗传多样性。
所有锥蝽对无形体科细菌、 、 均呈阴性。在36.4%（8/22）的锥蝽中检测到 DNA。在系统发育树中， 基因序列分为两个进化枝，分别与 和未培养的 sp. 克隆MYR - 283关系密切。所有 序列均与 的序列密切相关（同一性为98.75% - 100%）。 和 序列与一种公认的人类病原体 的序列关系最为密切，核苷酸相似性分别为98.70% - 100%和99.45% - 100%。
我们报告了在中国南方锥蝽中检测到 DNA。尽管样本量有限，但 DNA的高阳性检出率、基因序列与动物源性 物种序列的密切关系以及锥蝽在人类住所附近的分布，提示存在公共卫生风险。
