Hauke G, Epplen J T, Chluba J, Hollmann A, Peter H H
Medizinische Universitätsklinik, Freiburg, FRG.
J Clin Immunol. 1988 Sep;8(5):407-13. doi: 10.1007/BF00917157.
Human immunoglobulin (Ig) genes are rearranged in an ordered sequence of events during B-cell differentiation: starting at the IgH locus, a productive VHDJH rearrangement leads to the expression of mu chains. Light-chain gene rearrangements have been found in pre-B cells which express mu chains. In these cells rearrangements of Ig kappa light-chain genes precede that of lambda genes. In an IgD/lambda-producing plasmocytoma, however, we found an apparent exception to this rule: the kappa genes were not rearranged. Together with the observation that roughly 90% of human IgD plasmocytomas produce lambda light-chain proteins, the finding reported here leads us to suggest that lambda light-chain genes are rearranged preferentially in IgD-producing plasma cells. Ig gene rearrangement, isotype switch, and the phenomenon of isotypic and allelic exclusion are discussed with special reference to our findings.
人类免疫球蛋白（Ig）基因在B细胞分化过程中按有序的事件序列进行重排：从IgH基因座开始，有 productive VHDJH重排会导致μ链的表达。在表达μ链的前B细胞中发现了轻链基因重排。在这些细胞中，Igκ轻链基因的重排在λ基因之前。然而，在一个产生IgD/λ的浆细胞瘤中，我们发现了这条规则的一个明显例外：κ基因没有重排。结合大约90%的人类IgD浆细胞瘤产生λ轻链蛋白这一观察结果，此处报道的发现使我们提出，λ轻链基因在产生IgD的浆细胞中优先重排。本文特别参照我们的发现对Ig基因重排、同种型转换以及同种型和等位基因排斥现象进行了讨论。
