Yaoita Y, Matsunami N, Choi C Y, Sugiyama H, Kishimoto T, Honjo T
Nucleic Acids Res. 1983 Nov 11;11(21):7303-16. doi: 10.1093/nar/11.21.7303.
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of the JH-containing fragments have revealed the rearrangement from the preformed D-JH complex to the complete VH-D-JH gene, which was accompanied by the expression of the intra-cytoplasmic mu chain. In one case a JH segment downstream to the preformed D-JH was used to create a new VH-D-JH gene. Upon the D-JH and VH-D-JH rearrangements the intervening D segments were deleted from the chromosome. One of the expressed VH genes suffered from a large deletion of the 3' portion (including the 95th cysteine residue) of the VH segment. We discuss the possible mechanism of the allelic exclusion.
我们研究了来自单个阿贝尔逊鼠白血病病毒转化细胞的三个克隆中免疫球蛋白JH片段的组织情况。对含JH片段的克隆及核苷酸序列分析显示，从预先形成的D-JH复合体到完整的VH-D-JH基因发生了重排，同时伴有胞质内μ链的表达。在一个案例中，预先形成的D-JH下游的一个JH片段被用于创建一个新的VH-D-JH基因。在D-JH和VH-D-JH重排后，中间的D片段从染色体上缺失。其中一个表达的VH基因的VH片段3'端（包括第95个半胱氨酸残基）发生了大片段缺失。我们讨论了等位基因排斥的可能机制。
