Oduwole O A, Ameh S, Esu E B, Oringanje C M, Meremikwu J T, Meremikwu M M
Department of Medical Laboratory Science, College of Natural and Applied Sciences, Achievers University, Owo, Ondo State, Nigeria.
Department of Community Medicine, Faculty of Medicine, College of Medical Sciences, University of Calabar, Calabar, Nigeria; Department of Global Health and Population, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America.
Niger J Clin Pract. 2019 Aug;22(8):1078-1082. doi: 10.4103/njcp.njcp_66_19.
One of the major causes of anemia, defined as the reduction in the level of hemoglobin or red blood cells (RBCs) in the blood, in children in sub-Saharan Africa is malaria. Anemia is diagnosed by using either the hematocrit method or by measuring the hemoglobin concentration.
To evaluate the relationship and agreement between hemoglobin and three-fold conversion of hematocrit results of participants in a clinical trial.
This is a cross-sectional study that obtained data from a multi-center clinical trial that took place from 2007 to 2008 in public health facilities in Calabar, Nigeria. The hemoglobin and hematocrit results of 494 children who had ≥2000 parasite density recruited were pooled to evaluate the relationship and agreement between the two methods. The difference between the measures against the mean of the two measures was plotted according to the theory of Bland and Altman.
The mean age of the children was 34 months, with approximately equal number of boys and girls. The measured hemoglobin was lower than the calculated hemoglobin in 84.5% of the children. The result showed that lower the hemoglobin concentration, the higher the chances that the three-fold hematocrit conversion overestimates hemoglobin levels in the participants.
The three-fold hematocrit conversion of hemoglobin estimation is a less reliable method than the measured hemoglobin in anemic children in the study setting.
在撒哈拉以南非洲地区，儿童贫血（定义为血液中血红蛋白或红细胞水平降低）的主要原因之一是疟疾。贫血通过血细胞比容法或测量血红蛋白浓度来诊断。
评估一项临床试验参与者的血红蛋白与血细胞比容结果三倍转换之间的关系和一致性。
这是一项横断面研究，数据来自2007年至2008年在尼日利亚卡拉巴尔公共卫生设施中进行的一项多中心临床试验。汇总了494名寄生虫密度≥2000的儿童的血红蛋白和血细胞比容结果，以评估两种方法之间的关系和一致性。根据布兰德和奥特曼理论，绘制了测量值与两种测量值平均值之差的图表。
儿童的平均年龄为34个月，男孩和女孩数量大致相等。84.5%的儿童测量的血红蛋白低于计算的血红蛋白。结果表明，血红蛋白浓度越低，参与者中血细胞比容三倍转换高估血红蛋白水平的可能性越高。
在该研究环境中，对于贫血儿童，血红蛋白估计的血细胞比容三倍转换方法不如测量血红蛋白可靠。
