School of Medical Laboratory Sciences, Institute of Health, Jimma University, Jimma, Ethiopia.
Clinical Chemistry Laboratory Unit, Jimma Medical Center, Jimma University, Jimma, Ethiopia.
PLoS One. 2019 Aug 1;14(8):e0220740. doi: 10.1371/journal.pone.0220740. eCollection 2019.
Anemia is one of the common complications of pregnancy. Hemoglobin concentration, Hematocrit, and Red cell count are laboratory tests used to diagnose anemia. In are source poor setting, there is a practice of using three-fold converted Hematocrit. This study is designed to assess the association and acceptability of three-fold converted Hematocrit as compared to the standard Micro-hematocrit method, in pregnant women.
The cross-sectional study conducted from May 18 to June 12, 2018 involved 200 pregnant women who visited the Laboratory for a Hematocrit test. Three milliliter of venous blood was collected with EDTA tube to determine Hematocrit by the Micro-hematocrit method and Hemoglobin concentration measured by a HemoCue Hemoglobin B analyzer. A scatter plot, correlation coefficient, Bland and Altman plot, and Area under curve were employed to assess the agreement and acceptability of the calculated Hematocrit as compared to the standard Micro-hematocrit.
The correlation coefficient, Intraclass correlation coefficient and concordance correlation coefficient were 0.91, 0.94, and 0.89, respectively. The Bland and Altman plot showed a mean difference of 0.94 with the limit of agreement ranges from 0.6 to 1.3. The area under the receiver operating characteristics with cut-off point of Hematocrit <33% was 0.86. The sensitivity and specificity of the calculated method was 95.5% and 71.4%, respectively.
Generally there is excellent association between the two methods. The two methods were identical within inherent imprecision of both methods. Hence, the Hematocrit value, threefold calculated from the Hemoglobin was found to be acceptable to diagnose anemia in pregnant women.
贫血是妊娠的常见并发症之一。血红蛋白浓度、血细胞比容和红细胞计数是用于诊断贫血的实验室检测。在资源匮乏的情况下，存在使用三倍转换血细胞比容的做法。本研究旨在评估三倍转换血细胞比容与标准微量血细胞比容法的相关性和可接受性，用于诊断孕妇贫血。
这项横断面研究于 2018 年 5 月 18 日至 6 月 12 日进行，共纳入 200 名前往实验室进行血细胞比容检测的孕妇。用 EDTA 管采集 3 毫升静脉血，用微量血细胞比容法测定血细胞比容，用 HemoCue 血红蛋白 B 分析仪测定血红蛋白浓度。采用散点图、相关系数、Bland-Altman 图和曲线下面积来评估计算的血细胞比容与标准微量血细胞比容的一致性和可接受性。
相关系数、组内相关系数和一致性相关系数分别为 0.91、0.94 和 0.89。Bland-Altman 图显示平均差值为 0.94，一致性界限范围为 0.6 至 1.3。以血细胞比容<33%为截断值的受试者工作特征曲线下面积为 0.86。该计算方法的敏感性和特异性分别为 95.5%和 71.4%。
两种方法之间具有极好的相关性。两种方法在各自固有不精密度范围内完全一致。因此，从血红蛋白计算得出的血细胞比容值可用于诊断孕妇贫血。
