Başaran Nurşen, Duydu Yalçın, Üstündağ Aylin, Taner Gökçe, Aydin Sevtap, Anlar Hatice Gül, Yalçin Can Özgür, Bacanli Merve, Aydos Kaan, Atabekoğlu Cem Somer, Golka Klaus, Ickstadt Katja, Schwerdtle Tanja, Werner Matthias, Meyer Sören, Bolt Hermann M
Hacettepe University Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey.
Ankara University Faculty of Pharmacy, Department of Pharmaceutical Toxicology Ankara, Turkey.
Mutat Res Genet Toxicol Environ Mutagen. 2019 Jul;843:33-39. doi: 10.1016/j.mrgentox.2018.12.013. Epub 2018 Dec 23.
Industrial production and use of boron compounds have increased during the last decades, especially for the manufacture of borosilicate glass, fiberglass, metal alloys and flame retardants. This study was conducted in two districts of Balıkesir; Bandırma and Bigadic, which geographically belong to the Marmara Region of Turkey. Bandırma is the production and exportation zone for the produced boric acid and some borates and Bigadic has the largest B deposits in Turkey. 102 male workers who were occupationally exposed to boron from Bandırma and 110 workers who were occupationally and environmentally exposed to boron from Bigadic participated to our study. In this study the DNA damage in the sperm, blood and buccal cells of 212 males was evaluated by comet and micronucleus assays. No significant increase in the DNA damage in blood, sperm and buccal cells was observed in the residents exposed to boron both occupationally and environmentally (p = 0.861) for Comet test in the sperm samples, p = 0.116 for Comet test in the lymphocyte samples, p = 0.042 for micronucleus (MN) test, p = 0.955 for binucleated cells (BN), p = 1.486 for condensed chromatin (CC), p = 0.455 for karyorrhectic cells (KHC), p = 0.541 for karyolitic cells (KLY), p = 1.057 for pyknotic cells (PHC), p = 0.331 for nuclear bud (NBUD)). No correlations were seen between blood boron levels and tail intensity values of the sperm samples, lymphocyte samples, frequencies of MN, BN, KHC, KYL, PHC and NBUD. The results of this study came to the same conclusions of the previous studies that boron does not induce DNA damage even under extreme exposure conditions.
在过去几十年中，硼化合物的工业生产和使用有所增加，特别是在硼硅酸盐玻璃、玻璃纤维、金属合金和阻燃剂的制造方面。本研究在巴勒克埃西尔的两个区进行；班德尔马和比加迪克，这两个地区在地理上属于土耳其的马尔马拉地区。班德尔马是生产硼酸和一些硼酸盐的生产和出口区，比加迪克拥有土耳其最大的硼矿床。102名来自班德尔马的职业性接触硼的男性工人和110名来自比加迪克的职业性和环境性接触硼的工人参与了我们的研究。在本研究中，通过彗星试验和微核试验评估了212名男性精子、血液和颊细胞中的DNA损伤。在职业性和环境性接触硼的居民中，血液、精子和颊细胞中的DNA损伤没有显著增加（精子样本彗星试验p = 0.861，淋巴细胞样本彗星试验p = 0.116，微核（MN）试验p = 0.042，双核细胞（BN）p = 0.955，浓缩染色质（CC）p = 1.486，核溶解细胞（KHC）p = 0.455，核碎裂细胞（KLY）p = 0.541，固缩细胞（PHC）p = 1.057，核芽（NBUD）p = 0.331）。血液硼水平与精子样本、淋巴细胞样本的尾部强度值、MN、BN、KHC、KYL、PHC和NBUD的频率之间没有相关性。本研究的结果与先前的研究得出了相同的结论，即即使在极端暴露条件下，硼也不会诱导DNA损伤。
