Anlar Hatice Gul, Taner Gokce, Bacanli Merve, Iritas Servet, Kurt Turker, Tutkun Engin, Yilmaz Omer Hinc, Basaran Nursen
Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Hacettepe University, Ankara, Turkey.
Faculty of Natural Sciences, Architecture and Engineering, Department of Bioengineering, Bursa Technical University, Bursa, Turkey.
Mutagenesis. 2018 Feb 24;33(1):97-104. doi: 10.1093/mutage/gex016.
It is known that ceramic workers are potentially exposed to complex mixture of chemicals such as silica, inorganic lead, lime, beryllium and aluminum that can be associated with an increased risk of several diseases. All operations in the ceramic industries such as mixing, moulding, casting, shaking out and finishing jobs, have been associated with the higher exposure levels and in most of the silica-related industries, average overall exposure exceeded permissible exposure levels for respirable crystalline silica. The aim of this study was to evaluate the possible genotoxic damage in ceramic workers exposed to complex mixture of chemicals mainly crystalline silica. For this purpose, the blood and buccal epithelial cell samples were taken from the ceramic workers (n = 99) and their controls (n = 81). The genotoxicity was assessed by the alkaline comet assay in isolated lymphocytes and whole blood. Micronucleus (MN), binucleated (BN), pyknotic (PYC), condensed chromatin (CC), karyolytic (KYL), karyorrhectic (KHC) and nuclear bud (NBUD) frequencies in buccal epithelial cells and plasma 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) levels were also evaluated. In the study, 38 workers were diagnosed with silicosis, 9 workers were suspected to have silicosis, whereas 52 workers were found to be healthy. DNA damage in blood and lymphocytes; MN, CC + KHC, PYC frequencies in buccal epithelial cells and 8-oxodG levels in plasma were increased in workers compared to their controls. These results showed that occupational chemical mixture exposure in ceramic industry may cause genotoxic damage that can lead to important health problems in the workers.
众所周知，陶瓷工人可能会接触到复杂的化学物质混合物，如二氧化硅、无机铅、石灰、铍和铝，这些物质可能会增加患多种疾病的风险。陶瓷行业的所有操作，如混合、成型、铸造、脱模和精加工工作，都与较高的接触水平有关，并且在大多数与二氧化硅相关的行业中，平均总体接触量超过了可吸入结晶二氧化硅的允许接触水平。本研究的目的是评估接触主要为结晶二氧化硅的复杂化学物质混合物的陶瓷工人可能存在的遗传毒性损害。为此，采集了陶瓷工人（n = 99）及其对照组（n = 81）的血液和颊上皮细胞样本。通过碱性彗星试验评估分离淋巴细胞和全血中的遗传毒性。还评估了颊上皮细胞中的微核（MN）、双核（BN）、固缩核（PYC）、凝聚染色质（CC）、核溶解（KYL）、核碎裂（KHC）和核芽（NBUD）频率以及血浆8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代dG）水平。在该研究中，38名工人被诊断为矽肺病，9名工人疑似患有矽肺病，而52名工人被认定为健康。与对照组相比，工人血液和淋巴细胞中的DNA损伤；颊上皮细胞中的MN、CC + KHC、PYC频率以及血浆中的8-氧代dG水平均有所增加。这些结果表明，陶瓷行业的职业性化学物质混合物接触可能会导致遗传毒性损害，进而给工人带来重大健康问题。
