School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA, United States.
School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA, United States.
Food Microbiol. 2019 Dec;84:103272. doi: 10.1016/j.fm.2019.103272. Epub 2019 Jul 16.
One of the most important transmission routes of foodborne pathogens is through contaminated food contact surfaces. In this study, we investigated the survival and inactivation of Salmonella enterica serovar Typhimurium on frequently used food contact surfaces in household settings, including plastic cutting board (CB), formica laminate (LA), and stainless steel (SS) surfaces. S. Typhimurium at log (6 h), stationary (24 h), or long-term stationary (LTS) (14 days) phases were evaluated. Results showed that, at medium and high microbial loads, LTS phase cells had significantly higher survivor population compared to log and stationary phase cells at 24 h after inoculation (p < 0.05). Disinfection study showed that LTS phase cells were very resilient to sodium hypochlorite when the initial microbial load was high with disinfection efficacy ranging from 26 to 35%. Scanning electron microscopy of these surfaces at 24 h after inoculation with a high microbial load revealed an early biofilm structure.
食源性病原体最重要的传播途径之一是通过污染的食物接触面。本研究调查了食源性致病菌鼠伤寒沙门氏菌在家庭环境中常用的食物接触表面（包括塑料砧板、防火板和不锈钢表面）上的存活和失活情况。评估了处于对数（6h）、静止（24h）或长期静止（LTS）（14 天）阶段的鼠伤寒沙门氏菌。结果表明，在中高微生物负荷下，与接种后 24h 的对数和静止阶段细胞相比，LTS 阶段细胞的存活种群显著更高（p<0.05）。消毒研究表明，当初始微生物负荷较高时，LTS 阶段细胞对次氯酸钠具有很强的抵抗力，消毒效果在 26%至 35%之间。在高微生物负荷下接种 24h 后，对这些表面进行扫描电子显微镜检查显示出早期的生物膜结构。
