National Glycoengineering Research Center, and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, 250100, China.
Shandong Provincial Key Laboratory of Biomedical Polymers, Shandong Academy of Pharmaceutical Sciences, Jinan, 250101, China.
Carbohydr Polym. 2019 Nov 1;223:115106. doi: 10.1016/j.carbpol.2019.115106. Epub 2019 Jul 29.
Hyaluronic acid oligosaccharides (oHAs) have shown promising results in promoting vascular endothelial cell (EC) proliferation and endothelialization. To engineered develop tissue scaffold for promoting EC proliferation and vessel endothelialization, different sizes of oHAs were prepared and grafted onto collagen to improve the biological properties of the synthesized materials, especially in angiogenesis. Firstly, oHAs were successfully prepared and conjugated with collagen to construct glycosylated collagens by reductive amination. The glycosylated collagens were then electrospun into various nanofibrous structures and their morphology and hemocompatibility, as well as EC responses on these nanofibrous scaffolds, were studied to evaluate the potential for vascular tissue engineering. The results showed that the nanofibrous scaffolds grafted by oHAs promoted EC proliferation whereas high molecular weight HA inhibited proliferation. The scaffolds had no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular tissue scaffolds.
透明质酸低聚糖（oHAs）在促进血管内皮细胞（EC）增殖和内皮化方面显示出良好的效果。为了开发用于促进 EC 增殖和血管内皮化的组织支架，我们制备了不同大小的 oHAs 并将其接枝到胶原蛋白上，以改善合成材料的生物学特性，特别是在血管生成方面。首先，我们成功地制备了 oHAs，并通过还原胺化将其与胶原蛋白偶联，构建了糖基化胶原蛋白。然后，我们将糖基化胶原蛋白电纺成各种纳米纤维结构，并研究了它们的形态和血液相容性，以及这些纳米纤维支架上 EC 的反应，以评估其在血管组织工程中的应用潜力。结果表明，接枝 oHAs 的纳米纤维支架促进了 EC 的增殖，而高分子量 HA 则抑制了增殖。这些支架没有检测到溶血和凝血程度，表明它们有望成为工程化的血管组织支架。
