Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, Guangdong, People's Republic of China.
Materials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, People's Republic of China.
J Nanobiotechnology. 2021 Oct 30;19(1):349. doi: 10.1186/s12951-021-01091-0.
One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction.
组织工程血管移植物的主要挑战之一是由于内膜增生导致的再狭窄。本研究旨在开发一种支架材料，使其既能支持细胞生长，又能耐受生理条件，并保持颈总动脉模型的通畅性。通过顺序静电纺丝法制备管状透明质酸（HA）功能化胶原纳米纤维复合支架。管状复合支架具有良好控制的生物物理和生化信号，为血管内皮细胞（EC）的黏附和增殖提供了良好的基质，但在暴露于血液时能抵抗血小板黏附。来自 6 周龄兔子的颈动脉置换实验表明，内皮化在管腔表面的 HA/胶原纳米纤维复合支架移植物能够保持血管通畅。在取回时，复合支架保持了良好的结构完整性，其机械强度与天然动脉相当。本研究表明，静电纺丝支架与细胞相结合可能成为血管重建的一种替代假体移植物。
