费尔德斯增菌法对嗜血杆菌在自动细菌鉴定仪中进行药敏试验的影响
Effects of Fildes enrichment on antibiotic susceptibility testing of Haemophilus influenzae in the Autobac.
作者信息
Robinson D B, Schell R F, LeFrock J L, Chan J K
出版信息
Antimicrob Agents Chemother. 1979 Jul;16(1):98-100. doi: 10.1128/AAC.16.1.98.
The results of this investigation showed that the Autobac could be used to determine the susceptibility of Haemophilus influenzae to ampicillin, chloramphenicol, and tetracycline when eugonic broth was supplemented with 1.5% Fildes enrichment. An excellent correlation was obtained between the susceptibilities as determined by the agar dilution minimal inhibitory concentration method and the Autobac. For ampicillin all H. influenzae isolates with minimal inhibitory concentrations of </=1 mug/ml had light scattering indexes of >/=0.60. Those isolates with minimal inhibitory concentrations of >/=4 mug/ml had light scattering indexes of </=0.49. For chloramphenicol and tetracycline all isolates had minimal inhibitory concentrations of </=1 mug/ml and light scattering indexes of >/=0.71.
本次调查结果表明，当在营养肉汤中添加1.5%的费氏浸出液进行强化时，自动细菌鉴定仪可用于测定流感嗜血杆菌对氨苄西林、氯霉素和四环素的敏感性。通过琼脂稀释法测定的最低抑菌浓度与自动细菌鉴定仪所测定的敏感性之间具有极佳的相关性。对于氨苄西林，所有最低抑菌浓度≤1μg/ml的流感嗜血杆菌分离株的光散射指数≥0.60。那些最低抑菌浓度≥4μg/ml的分离株的光散射指数≤0.49。对于氯霉素和四环素，所有分离株的最低抑菌浓度≤1μg/ml且光散射指数≥0.71。
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