Gebreyohannes Gebreselema, Nyerere Andrew, Bii Christine, Sbhatu Desta B
Department of Biological and Chemical Engineering, Mekelle Institute of Technology, Mekelle University, Ethiopia.
Molecular Biology and Biotechnology, Pan African University, Institute for Basic Sciences, Technology, and Innovation, Nairobi, Kenya.
ScientificWorldJournal. 2019 Jul 24;2019:7357048. doi: 10.1155/2019/7357048. eCollection 2019.
Mushrooms produce a variety of bioactive compounds that are known to have a potential source of antioxidant and antimicrobial properties. Natural antioxidants can protect against free radicals without any side effects. The purpose of this study was to evaluate the antioxidant and antimicrobial activities of and extracts. Specimens of and spp. were collected from Kakamega National Reserve Forest in Kenya. Specimens were identified, extracted, and screened for their antioxidant and antimicrobial activities using stable free radical DPPH and colorimetric bioassay methods, respectively. The antimicrobial activity of the extracts was tested against , , MRSA, , and . The maximum scavenging activity of hot water extract of spp. was observed at 70.4% with the IC value of 40 g/mL. Of the three extracts of spp., 70% ethanol extract has shown the highest scavenging activity (63%) with the IC value of 50 g/mL. Chloroform and hot water extracts of have shown statistically significantly different antifungal activities against ( = 2, F = 22.49, ≤ 0.05). Of all the organisms, was highly susceptible to 70% ethanol and hot water extracts of spp. with minimum inhibitory concentration values of 0.67±0.29 mg/mL. and were the most susceptible and resistant bacteria to the hot water extract, respectively. In conclusion, the extracts of spp. and spp. have shown promising antimicrobial and antioxidant activities.
蘑菇能产生多种生物活性化合物，这些化合物已知具有抗氧化和抗菌特性的潜在来源。天然抗氧化剂可以抵御自由基且无任何副作用。本研究的目的是评估[物种名1]和[物种名2]提取物的抗氧化和抗菌活性。[物种名1]和[物种名2]的标本采集自肯尼亚的卡卡梅加国家保护区森林。标本经过鉴定、提取，并分别使用稳定自由基DPPH和比色生物测定法对其抗氧化和抗菌活性进行筛选。提取物的抗菌活性针对[细菌名1]、[细菌名2]、耐甲氧西林金黄色葡萄球菌（MRSA）、[细菌名3]和[细菌名4]进行测试。[物种名1]热水提取物的最大清除活性在70.4%时观察到，IC值为40μg/mL。在[物种名2]的三种提取物中，70%乙醇提取物显示出最高的清除活性（63%），IC值为50μg/mL。[物种名3]的氯仿和热水提取物对[真菌名1]显示出统计学上显著不同的抗真菌活性（自由度=2，F=22.49，P≤0.05）。在所有测试的生物体中，[细菌名5]对[物种名1]的70%乙醇和热水提取物高度敏感，最低抑菌浓度值为0.67±0.29mg/mL。[细菌名6]和[细菌名7]分别是对热水提取物最敏感和最耐药的细菌。总之，[物种名1]和[物种名2]的提取物显示出有前景的抗菌和抗氧化活性。
