Wu Siyu, Zhang Junsheng, Huang Jianni, Li Weiqiang, Liu Zhiting, He Zhuoliang, Chen Zuxian, He Wanting, Zhao Bingbing, Qin Zhifeng, Jiao Peirong, Liao Ming
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Shenzhen Academy of Inspection and Quarantine, Shenzhen, China.
Front Microbiol. 2019 Aug 2;10:1782. doi: 10.3389/fmicb.2019.01782. eCollection 2019.
Clade 2.3.4.4 H5 avian influenza viruses (AIVs) are widely prevalent and of significant concern to the poultry industry and public health in China. Nowadays, the clade 2.3.4.4 H5N6 virus has become a dominant AIV subtype among domestic ducks in southern China. We found that waterfowl-origin clade 2.3.4.4 H5N6 viruses (A/goose/Guangdong/16568/2016, GS16568 and A/duck/Guangdong/16873/2016, DK16873) isolated from southern China in 2016 could replicate in multiple organs of inoculated ducks. DK16873 virus caused mild infections and killed 2/5 of inoculated ducks, and GS16568 virus did not kill inoculated ducks. In addition, the two viruses could be transmitted via direct contact between ducks. DK16873 and GS16568 viruses killed 2/5 and 1/5 of contact ducks, respectively. Furthermore, ducks inoculated with the two H5N6 viruses exhibited different expressions of immune-related genes in their lungs. The expression of RIG-I, TLR3 and IL6 was significantly upregulated at 12 h post-inoculation (HPI) and most of the tested immune-related genes were significantly upregulated at 3 days post-inoculation (DPI). Notably, the expression of RIG-I and IL-6 in response to DK16873 virus was significantly higher than for GS16568 virus at 12 HPI and 3 DPI. Our research have provided helpful information about the pathogenicity, transmission and immune-related genes expression in ducks infected with new H5N6 AIVs.
2.3.4.4分支H5禽流感病毒（AIV）在中国广泛流行，对家禽业和公共卫生构成重大威胁。目前，2.3.4.4分支H5N6病毒已成为中国南方家鸭中占主导地位的AIV亚型。我们发现，2016年从中国南方分离的水禽源2.3.4.4分支H5N6病毒（A/鹅/广东/16568/2016，GS16568和A/鸭/广东/16873/2016，DK16873）可在接种鸭的多个器官中复制。DK16873病毒引起轻度感染，导致2/5的接种鸭死亡，而GS16568病毒未导致接种鸭死亡。此外，这两种病毒可通过鸭之间的直接接触传播。DK16873和GS16568病毒分别导致2/5和1/5的接触鸭死亡。此外，接种这两种H5N6病毒的鸭在肺部表现出不同的免疫相关基因表达。RIG-I、TLR3和IL6的表达在接种后12小时（HPI）显著上调，大多数测试的免疫相关基因在接种后3天（DPI）显著上调。值得注意的是，在12 HPI和3 DPI时，DK16873病毒刺激下RIG-I和IL-6的表达显著高于GS16568病毒。我们的研究为感染新型H5N6 AIV的鸭的致病性、传播和免疫相关基因表达提供了有用信息。
