Sood Kritika, Kaur Jasreen, Singh Harpreet, Kumar Arya Shailendra, Khatri Madhu
Department of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, India.
Center for Nanoscience and Nanotechnology, Panjab University, Chandigarh, 160014, India.
Toxicol Rep. 2019 Jul 30;6:768-781. doi: 10.1016/j.toxrep.2019.07.009. eCollection 2019.
Engineered nanomaterials consisting of multiple nanoparticles (NPs) are finding their use in fields as wide and diverse as medicine, environment, cosmetics, energy and electronics. However, health and environmental impacts of these NPs need to be discerned individually to understand their true toxicity. Due to the promising application of upcoming material like GO-ZnO nanocomposite, the toxicity of ZnO and GO NPs was evaluated and compared individually in our study. This study compares the toxicity of Graphene Oxide (GO) NPs and Zinc Oxide (ZnO) NPs synthesized by Green method and Chemical method on . The GO, Chemical ZnO and Green ZnO NPs were synthesized and characterized using SEM, HR-TEM, FT-IR, UV-vis, EDX, XRD and DLS studies. NPs were comparatively analyzed for their cytotoxic and neurotoxic behaviors using different assays like MTT assay, mortality rate, larval crawling and climbing assay, total protein content analysis for evaluating the toxic potential of each of these NPs at different concentrations of use. Green ZnO were found to be least cytotoxic while Chemical ZnO caused the most cell damage. GO were found to have intermediary cytotoxicity. However, a different trend was observed with neurotoxicity wherein Green ZnO reportedly affected the neuromuscular coordination the most, while GO was found to have the least affect. This study provided insights into the different toxic effects caused by GO and ZnO NPs on as well as comparative toxic effects of Chemical vs Green ZnO NPs.
由多个纳米颗粒（NPs）组成的工程纳米材料正在医学、环境、化妆品、能源和电子等广泛多样的领域中得到应用。然而，需要分别了解这些纳米颗粒对健康和环境的影响，以了解它们的真正毒性。由于即将出现的材料如氧化石墨烯-氧化锌纳米复合材料具有广阔的应用前景，因此在我们的研究中分别评估和比较了氧化锌纳米颗粒和氧化石墨烯纳米颗粒的毒性。本研究比较了通过绿色方法和化学方法合成的氧化石墨烯（GO）纳米颗粒和氧化锌（ZnO）纳米颗粒对……的毒性。通过扫描电子显微镜（SEM）、高分辨率透射电子显微镜（HR-TEM）、傅里叶变换红外光谱（FT-IR）、紫外可见光谱（UV-vis）、能谱分析（EDX）、X射线衍射（XRD）和动态光散射（DLS）研究对GO、化学法制备的氧化锌和绿色法制备的氧化锌纳米颗粒进行了合成和表征。使用不同的检测方法，如MTT检测、死亡率、幼虫爬行和攀爬检测、总蛋白含量分析，对纳米颗粒在不同使用浓度下的细胞毒性和神经毒性行为进行了比较分析，以评估每种纳米颗粒的潜在毒性。结果发现绿色法制备的氧化锌细胞毒性最小，而化学法制备的氧化锌对细胞的损伤最大。氧化石墨烯具有中等细胞毒性。然而，在神经毒性方面观察到了不同的趋势，据报道绿色法制备的氧化锌对神经肌肉协调的影响最大，而氧化石墨烯的影响最小。这项研究深入了解了氧化石墨烯和氧化锌纳米颗粒对……造成的不同毒性作用，以及化学法制备的氧化锌和绿色法制备的氧化锌纳米颗粒的比较毒性作用。
